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Literature summary extracted from

  • Stocchi, V.; Magnani, M.; Novelli, G.; Dacha, M.; Fornaini, G.
    Pig red blood cell hexokinase: Evidence for the presence of hexokinase types II and III, and their purification and characterization (1983), Arch. Biochem. Biophys., 226, 365-376.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.1 D-glucose, D-fructose, D-mannose and high concentrations of glycerol, 10-15% v/v, stabilize the purified enzyme Sus scrofa
2.7.1.1 sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 D-glucose higher than 0.4 mM; inhibits hexokinase III Sus scrofa
2.7.1.1 D-glucose 6-phosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.014
-
 D-mannose hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 0.015
-
 D-glucose hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 0.033
-
 2-deoxy-D-glucose hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 0.15
-
 D-glucose hexokinase II, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 0.2
-
 D-glucosamine hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 0.3
-
 ATP hexokinase II, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 0.5
-
 ATP hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 1.5
-
 D-fructose hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.7.1.1 2.5
-
 ITP hexokinase III, at 30°C, pH 8.1 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ required Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 100000
-
 gel filtration Sus scrofa
2.7.1.1 100000
-
 hexokinases II and III Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Sus scrofa
-
 adult pigs
-
2.7.1.1 Sus scrofa
-
 2 enzyme forms: hexokinase type II and type III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 hexokinase III: 290000fold, hexokinase II: partial, 39160fold Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 erythrocyte hexokinase types II and III, hexokinase III is the predominant form in adult pig erythrocytes, hexokinase II in newborn pig erythrocytes Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 0.25
-
 hexokinase type II, at 37°C, pH 8.1 Sus scrofa
2.7.1.1 191.5
-
 hexokinase type III, at 37°C, pH 8.1 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose hexokinase III: 151% of the activity with glucose Sus scrofa ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 CTP + D-glucose
-
 Sus scrofa CDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP hexokinase III: 12.6% of the activity with glucose Sus scrofa ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP hexokinase III: 35% of the activity with glucose Sus scrofa ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Sus scrofa ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP hexokinase III: 85% of the activity with glucose Sus scrofa ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose hexokinase III: 24% of the activity with glucose Sus scrofa IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose MgITP Sus scrofa IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information not: UTP Sus scrofa ?
-
 ?
2.7.1.1 additional information not: GTP Sus scrofa ?
-
 ?
2.7.1.1 additional information not: CTP Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 100000, hexokinase III, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 37
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.1 44
-
 5 mM sodium phosphate buffer, pH 7.5, 0.25 mM glucose, 10% v/v glycerol, 1 mM dithiothreitol, inactivation as a function of time, hexokinase type III is more stable than hexokinase type II Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8
-
 hexokinase type III Sus scrofa
2.7.1.1 9
-
 hexokinase type II Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Sus scrofa hexokinase III, isoelectric focusing 4.4 4.3
2.7.1.1 Sus scrofa hexokinase II, isoelectric focusing
-
 4.8