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Literature summary extracted from

  • Renz, A.; Stitt, M.
    Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers (1993), Planta, 190, 166-175.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP isoenzymes HK1 and HK2, competitive to ATP, 0.5 mM: 4-5fold inhibition Solanum tuberosum
2.7.1.1 AMP weak Solanum tuberosum
2.7.1.1 D-glucose 6-phosphate isoenzyme HK1, not HK2, noncompetitive to glucose Solanum tuberosum
2.7.1.1 UDP weak Solanum tuberosum
2.7.1.1 UMP very weak Solanum tuberosum
2.7.1.4 ADP competitive to ATP Solanum tuberosum
2.7.1.4 D-fructose substrate inhibition Solanum tuberosum
2.7.1.4 D-fructose 6-phosphate
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.035
-
 D-glucose HK3 Solanum tuberosum
2.7.1.1 0.038
-
 D-mannose HK3 Solanum tuberosum
2.7.1.1 0.041
-
 D-glucose HK1 Solanum tuberosum
2.7.1.1 0.052
-
 D-mannose HK1 Solanum tuberosum
2.7.1.1 0.09
-
 ATP HK1 Solanum tuberosum
2.7.1.1 0.13
-
 D-glucose HK2 Solanum tuberosum
2.7.1.1 0.28
-
 ATP HK2 Solanum tuberosum
2.7.1.1 0.29
-
 D-mannose HK2 Solanum tuberosum
2.7.1.1 0.56
-
 ATP HK3 Solanum tuberosum
2.7.1.1 8.7
-
 D-fructose HK3 Solanum tuberosum
2.7.1.1 11
-
 D-fructose HK1 Solanum tuberosum
2.7.1.1 22
-
 D-fructose HK2 Solanum tuberosum
2.7.1.4 0.025
-
 ATP FK 2, pH 8 Solanum tuberosum
2.7.1.4 0.026
-
 ATP FK 1, pH 8 Solanum tuberosum
2.7.1.4 0.064
-
 D-fructose FK 1, pH 8 Solanum tuberosum
2.7.1.4 0.09
-
 D-fructose FK 2, pH 8 Solanum tuberosum
2.7.1.4 0.1
-
 D-fructose FK 3, pH 8 Solanum tuberosum
2.7.1.4 0.24
-
 ATP FK 3, pH 8 Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ Mg2+-dependent Solanum tuberosum
2.7.1.4 Mg2+ maximal activation when total Mg2+ concentration is about 1.5 mM higher than the concentration of ATP Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-glucose + ATP Solanum tuberosum glucose metabolism D-glucose 6-phosphate + ADP
-
 ?
2.7.1.4 ATP + D-fructose Solanum tuberosum
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Solanum tuberosum
-
 cv. Desiree, 3 isoenzymes: HK1, HK2, HK3
-
2.7.1.4 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 partial Solanum tuberosum
2.7.1.4 FK1, FK2, FK3 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 tuber developing Solanum tuberosum
-
2.7.1.4 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-fructose + ATP HK1: 4% of the activity with glucose, HK2: 3% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP HK3: 5% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Solanum tuberosum ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP glucose metabolism Solanum tuberosum D-glucose 6-phosphate + ADP
-
 ?
2.7.1.1 D-mannose + ATP HK1: 53% of the activity with glucose, HK2: 22% of the activity with glucose, HK3: 45% of the activity with glucose Solanum tuberosum ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 GTP + D-glucose
-
 Solanum tuberosum GDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 UTP + D-glucose
-
 Solanum tuberosum UDP + D-glucose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose
-
 Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose higher affinity for ATP than for other NTPs Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 CTP + D-fructose
-
 Solanum tuberosum CDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose
-
 Solanum tuberosum GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 additional information no substrate: D-mannose Solanum tuberosum ?
-
 ?
2.7.1.4 additional information no substrate: D-glucose Solanum tuberosum ?
-
 ?
2.7.1.4 UTP + D-fructose
-
 Solanum tuberosum UDP + D-fructose 6-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.3 8.2 HK 1 Solanum tuberosum
2.7.1.1 7.5 9 HK 2 Solanum tuberosum
2.7.1.1 8
-
 assay at Solanum tuberosum
2.7.1.4 7.8 8.5
-
 Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 6.7 9 pH 6.7: about 40% of activity maximum, pH 9: about 70% of activity maximum, isoenzyme HK1 Solanum tuberosum
2.7.1.4 6.7 9.3 pH 6.7: about 50% of activity maximum, pH 9.3: about 60% of activity maximum Solanum tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Solanum tuberosum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.04
-
 ADP HK1 Solanum tuberosum
2.7.1.1 0.108
-
 ADP HK2 Solanum tuberosum
2.7.1.1 4.1
-
 D-glucose 6-phosphate HK1, at pH 7 Solanum tuberosum
2.7.1.4 1.3
-
 D-fructose 6-phosphate FK 1, pH 8 Solanum tuberosum
2.7.1.4 5.7
-
 D-fructose FK 1, pH 8 Solanum tuberosum
2.7.1.4 6
-
 D-fructose FK 2, pH 8 Solanum tuberosum
2.7.1.4 21
-
 D-fructose FK 3, pH 8 Solanum tuberosum