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Literature summary extracted from

  • Renz, A.; Merlo, L.; Stitt, M.
    Partial purification from potato tubers of three fructokinases and three hexokinases which show differing organ and developmental specificity (1993), Planta, 190, 156-165.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 66000
-
 HK1 and HK2, gel filtration Solanum tuberosum
2.7.1.4 102000
-
 FK 1, gel filtration Solanum tuberosum
2.7.1.4 105000
-
 FK 1, gel filtration Solanum tuberosum
2.7.1.4 118000
-
 FK 1, gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-glucose + ATP Solanum tuberosum organ- and development-specific changes in the abundance of the various enzyme forms contribute to the regulation of hexose metabolism D-glucose 6-phosphate + ADP
-
 ?
2.7.1.4 ATP + D-fructose Solanum tuberosum
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Solanum tuberosum
-
 cv. Desiree, 3 isoenzymes: HK1, HK2, HK3
-
2.7.1.4 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 3 isoenzymes: HK1: 140fold, HK2: 40fold, HK3: 25fold Solanum tuberosum
2.7.1.1 partial Solanum tuberosum
2.7.1.4 FK1, FK2, FK3 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 tuber developing Solanum tuberosum
-
2.7.1.1 tuber organ- and development-specific changes in the abundance of the 3 isoenzymes Solanum tuberosum
-
2.7.1.4 leaf high activity: isoenzyme FK3, very low activity: FK1, FK2 Solanum tuberosum
-
2.7.1.4 tuber high activity: isoenzyme FK1, FK2, very low activity: FK3 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 0.06
-
 HK3, at pH 8 Solanum tuberosum
2.7.1.1 0.72
-
 HK2, at pH 8 Solanum tuberosum
2.7.1.1 0.84
-
 HK1, at pH 8 Solanum tuberosum
2.7.1.4 0.8
-
 FK 3 Solanum tuberosum
2.7.1.4 10.7
-
 FK 1 Solanum tuberosum
2.7.1.4 20.2
-
 FK 2 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
2.7.1.1 -180°C, 2 months, stable Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-fructose + ATP HK3: 16% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP HK1: 4% of the activity with glucose, HK2: 3% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Solanum tuberosum ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP organ- and development-specific changes in the abundance of the various enzyme forms contribute to the regulation of hexose metabolism Solanum tuberosum D-glucose 6-phosphate + ADP
-
 ?
2.7.1.4 ATP + D-fructose
-
 Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 additional information no substrate: D-glucose Solanum tuberosum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8
-
 assay at Solanum tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Solanum tuberosum