EC Number | General Stability | Organism |
---|---|---|
2.7.1.1 | stability depends on the presence of 1 mM D-glucose, 3 mM mercaptoethanol, and 9% v/v glycerol | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.1 | Cl- | reversible, 0.25 M NaCl or KCl: 50% inhibition, at a comparable concentration of LiCl: 30% inhibition | Homo sapiens | |
2.7.1.1 | I- | at about 0.25 M KI, 70% inhibition, reversible | Homo sapiens | |
2.7.1.1 | KSCN | at about 0.25 M, 90% inhibition, reversible | Homo sapiens | |
2.7.1.1 | NO3- | at about 0.25 M NaNO3, 40% inhibition, reversible | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.1 | 0.06 | - |
D-glucose | hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 0.1 | - |
D-mannose | at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 0.1 | - |
D-mannose | hexokinase I | Homo sapiens | |
2.7.1.1 | 0.5 | - |
2-deoxy-D-glucose | hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 0.6 | - |
D-glucosamine | hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 0.6 | - |
ATP | MgATP, hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 5 | - |
UTP | MgUTP2-, hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 5 | - |
CTP | MgCTP2-, hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 11.4 | - |
D-fructose | hexokinase I, at 37°C, pH 8.1 | Homo sapiens | |
2.7.1.1 | 16.6 | - |
ITP | MgITP2-, hexokinase I, at 37°C, pH 8.1 | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.1 | mitochondrion | 15% of total placenta hexokinase activity, only hexokinase I | Homo sapiens | 5739 | - |
2.7.1.1 | soluble | 85% of total placenta hexokinase activity is soluble, of which 70% is hexokinase type I and 30% hexokinase type II | Homo sapiens | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.1 | 103000 | - |
1 * 103000 or 1 * 112000, 2 subtypes of hexokinase type I, SDS-PAGE | Homo sapiens |
2.7.1.1 | 112000 | - |
1 * 103000 or 1 * 112000, 2 subtypes of hexokinase type I, SDS-PAGE | Homo sapiens |
2.7.1.1 | 118000 | - |
both subtypes of hexokinase type I, gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.1 | D-glucose + ATP | Homo sapiens | glucose metabolism | D-glucose 6-phosphate + ADP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.1 | Homo sapiens | - |
2 subtypes of hexokinase type I | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.1 | soluble hexokinase type I with 2 unseparated subtypes, 11600fold | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.1 | placenta | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.1 | 192.5 | - |
at 37°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.1 | ATP + 2-deoxy-D-glucose | hexokinase I: as effective as glucose | Homo sapiens | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
2.7.1.1 | CTP + D-glucose | MgCTP, hexokinase I: 5% of the activity with ATP | Homo sapiens | CDP + D-glucose 6-phosphate | - |
? | |
2.7.1.1 | D-fructose + ATP | hexokinase I: 130% of the activity with glucose | Homo sapiens | ADP + D-fructose 6-phosphate | - |
? | |
2.7.1.1 | D-glucosamine + ATP | hexokinase I: 40% of the activity with glucose | Homo sapiens | ADP + D-glucosamine 6-phosphate | - |
? | |
2.7.1.1 | D-glucose + ATP | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.1 | D-glucose + ATP | glucose metabolism | Homo sapiens | D-glucose 6-phosphate + ADP | - |
? | |
2.7.1.1 | D-mannose + ATP | hexokinase I: 106% of the activity with glucose | Homo sapiens | ADP + D-mannose 6-phosphate | - |
? | |
2.7.1.1 | ITP + D-glucose | MgITP | Homo sapiens | IDP + D-glucose 6-phosphate | - |
? | |
2.7.1.1 | ITP + D-glucose | hexokinase I: 9% of the activity with ATP | Homo sapiens | IDP + D-glucose 6-phosphate | - |
? | |
2.7.1.1 | additional information | not: GTP | Homo sapiens | ? | - |
? | |
2.7.1.1 | UTP + D-glucose | hexokinase I: 5% of the activity with ATP | Homo sapiens | UDP + D-glucose 6-phosphate | - |
? | |
2.7.1.1 | UTP + D-glucose | MgUTP | Homo sapiens | UDP + D-glucose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.1 | monomer | 1 * 103000 or 1 * 112000, 2 subtypes of hexokinase type I, SDS-PAGE | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.1 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.1 | 8 | 8.5 | hexokinase I | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.1 | ATP | - |
Homo sapiens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.1.1 | Homo sapiens | isoelectric focusing | - |
5.9 |