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Literature summary extracted from

  • Stocchi, V.; Magnani, M.; Canestrari, F.; Dacha, M.; Fornaini, G.
    Rabbit red blood cell hexokinase. Evidence for two distinct forms, and their purification and characterization from reticulocytes (1981), J. Biol. Chem., 256, 7856-7862.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.1 D-glucose or D-fructose, 5 mM, stabilizes Oryctolagus cuniculus
2.7.1.1 glycerol, 9-15% v/v, stabilizes Oryctolagus cuniculus
2.7.1.1 sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.04
-
 D-glucose reticulocyte hexokinase Ia Oryctolagus cuniculus
2.7.1.1 0.04
-
 D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 0.1
-
 D-mannose hexokinase Ia Oryctolagus cuniculus
2.7.1.1 0.125
-
 D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 0.125
-
 D-glucose reticulocyte hexokinase Ib Oryctolagus cuniculus
2.7.1.1 0.15
-
 D-mannose hexokinase Ib Oryctolagus cuniculus
2.7.1.1 0.5
-
 ATP MgATP2- Oryctolagus cuniculus
2.7.1.1 0.5
-
 ATP reticulocyte hexokinase Ia and Ib, at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 0.85
-
 D-glucosamine hexokinase Ia Oryctolagus cuniculus
2.7.1.1 0.9
-
 2-deoxy-D-glucose hexokinase Ia Oryctolagus cuniculus
2.7.1.1 1.3
-
 D-glucosamine hexokinase Ib Oryctolagus cuniculus
2.7.1.1 2
-
 2-deoxy-D-glucose hexokinase Ib Oryctolagus cuniculus
2.7.1.1 4
-
 D-fructose hexokinase Ia Oryctolagus cuniculus
2.7.1.1 4.5
-
 ITP MgITP2-, hexokinase Ib Oryctolagus cuniculus
2.7.1.1 12
-
 ITP MgITP2-, hexokinase Ia Oryctolagus cuniculus
2.7.1.1 25
-
 D-fructose hexokinase Ib Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.1 soluble hexokinase Ia and Ib Oryctolagus cuniculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ required Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 104000
-
 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE Oryctolagus cuniculus
2.7.1.1 105000
-
 reticulocyte hexokinase Ia and Ib, gel filtration, sucrose density gradient sedimentation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Oryctolagus cuniculus
-
 hexokinase Ia and Ib
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 erythrocyte enzyme and reticulocyte hexokinase Ia and Ib Oryctolagus cuniculus
2.7.1.1 hexokinase Ia: 55460fold, hexokinase Ib: 51260fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 reticulocyte hexokinase Ia and Ib Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 144
-
 pH 8.1 Oryctolagus cuniculus
2.7.1.1 144
-
 reticulocyte, hexokinase Ib, at 37°C Oryctolagus cuniculus
2.7.1.1 155
-
 pH 8.1 Oryctolagus cuniculus
2.7.1.1 155
-
 reticulocyte, hexokinase Ia, at 37°C Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.1 -25°C, over a period of months, stable Oryctolagus cuniculus
2.7.1.1 37°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ib is more stable than hexokinase Ia Oryctolagus cuniculus
2.7.1.1 4°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ia is more stable than hexokinase Ib Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose hexokinase Ia: 65% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 CTP + D-glucose
-
 Oryctolagus cuniculus CDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP hexokinase Ia: 69% of the activity with glucose, hexokinase Ib: 42% of the activity with glucose Oryctolagus cuniculus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP hexokinase Ia: 35% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP MgATP2- Oryctolagus cuniculus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 104-105% of the activity with glucose Oryctolagus cuniculus ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose 30% of the activity with ATP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose MgITP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information not: UTP Oryctolagus cuniculus ?
-
 ?
2.7.1.1 additional information not: N-acetyl-D-glucosamine Oryctolagus cuniculus ?
-
 ?
2.7.1.1 additional information not: D-galactose Oryctolagus cuniculus ?
-
 ?
2.7.1.1 additional information not: GTP Oryctolagus cuniculus ?
-
 ?
2.7.1.1 additional information not: CTP Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8 8.2
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Oryctolagus cuniculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Oryctolagus cuniculus reticulocyte hexokinase Ib
-
 5.7
2.7.1.1 Oryctolagus cuniculus isoelectric focusing
-
 5.7
2.7.1.1 Oryctolagus cuniculus hexokinase Ia, isoelectric focusing
-
 6.2