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Literature summary extracted from

  • Livneh, Z.; Elad, D.; Sperling, J.
    Enzymatic insertion of purine bases into depurinated DNA in vitro (1979), Proc. Natl. Acad. Sci. USA, 76, 1089-1093.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.6.99.1 complete inactivation after 15 min at 60°C Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.99.1 Caffeine
-
Escherichia coli
2.6.99.1 additional information inhibited by high salt concentration e.g. 1 M NaCl Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.99.1 cytoplasm
-
Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.99.1 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.99.1 additional information Escherichia coli may represent a step in a repair mechanism, i.e. base-insertion repair, whereby apurinic sites, which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination, are directly filled with the correct missing purine base ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.99.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.99.1 dATP + depurinated DNA closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate Escherichia coli deoxyribose triphosphate + purinated DNA
-
?
2.6.99.1 dGTP + depurinated DNA closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate Escherichia coli deoxyribose triphosphate + purinated DNA
-
?
2.6.99.1 dTTP + depurinated DNA lower activity than that of dATP and dGTP, incorporation is supressed in the presence of dATP Escherichia coli deoxyribose triphosphate + purinated DNA
-
?
2.6.99.1 additional information specific insertion of adenine into adenine apurinic sites and guanine into guanine apurinic sites Escherichia coli ?
-
?
2.6.99.1 additional information enzyme does not act on depurinated single-stranded DNA Escherichia coli ?
-
?
2.6.99.1 additional information may represent a step in a repair mechanism, i.e. base-insertion repair, whereby apurinic sites, which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination, are directly filled with the correct missing purine base Escherichia coli ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.99.1 37
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.99.1 7.4
-
assay at Escherichia coli