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Literature summary extracted from

  • Deutsch, W.A.; Linn, S.
    DNA binding activity from cultured human fibrolasts that is specific for partially depurinated DNA and that inserts purines into apurinic sites (1979), Proc. Natl. Acad. Sci. USA, 76, 141-144.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.6.99.1 sensitive to freezing and heating, extremely sensitive to dialysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.99.1 Caffeine 20-80% inhibition when present at 0.1-1 mM Homo sapiens
2.6.99.1 EDTA complete inhibition at 1.3 mM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.99.1 cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.99.1 K+ specific requirement, cannot be replaced by NH4+, Na+, Mg2+ or Mn2+ Homo sapiens
2.6.99.1 Li+ supports activity to a small degree Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.99.1 120000
-
 glycerol density gradient centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.99.1 additional information Homo sapiens possible alternative pathway for DNA repair ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.99.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.99.1 partial, using DEAE-cellulose and phosphocellulose chromatography followed by glycerol gradient centrifugation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.99.1 fibroblast cultured Homo sapiens
-
2.6.99.1 HeLa cell
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.6.99.1 unstable, activity retained for less than 1 week after purification, addition of phenylmethylsulfonyl fluoride or N-alpha-p-tosyl-L-lysine chloromethylketone hydrochloride can stabilize enzymatic fractions Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.99.1 dATP + depurinated DNA closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate Homo sapiens deoxyribose triphosphate + purinated DNA
-
 ?
2.6.99.1 dATP + depurinated poly(dA-dT) dGTP cannot act as substrate Homo sapiens deoxyribose triphosphate + purinated poly(dA-dT)
-
 ?
2.6.99.1 dGTP + depurinated DNA closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate Homo sapiens deoxyribose triphosphate + purinated DNA
-
 ?
2.6.99.1 dGTP + depurinated poly(dG-dC) dATP cannot act as substrate Homo sapiens deoxyribose triphosphate + purinated poly(dG-dC)
-
 ?
2.6.99.1 additional information specific insertion of adenine into adenine apurinic sites and guanine into guanine apurinic sites Homo sapiens ?
-
 ?
2.6.99.1 additional information possible alternative pathway for DNA repair Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.99.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.99.1 7.5
-
-
 Homo sapiens