Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Laue, H.; Cook, A.M.
    Biochemical and molecular characterization of taurine:pyruvate transaminase from the anaerobe Bilophila wadsworthia (2000), Eur. J. Biochem., 267, 6841-6848.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.77 DNA sequence determination and analysis Bilophila wadsworthia

General Stability

EC Number General Stability Organism
2.6.1.77 glycerol stabilizes Bilophila wadsworthia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.77 0.82
-
 pyruvate pH 9.0, 35°C Bilophila wadsworthia
2.6.1.77 7.1
-
 taurine pH 9.0, 35°C Bilophila wadsworthia
2.6.1.77 8.1
-
 Hypotaurine pH 9.0, 35°C Bilophila wadsworthia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.77 51000
-
 4 * 51000, SDS-PAGE Bilophila wadsworthia
2.6.1.77 197000
-
 gel filtration Bilophila wadsworthia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.77 taurine + pyruvate Bilophila wadsworthia initial step in anaerobic taurine degradation L-alanine + 2-sulfoacetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.77 Bilophila wadsworthia Q9APM5 RZATAU
-
2.6.1.77 Bilophila wadsworthia RZATAU Q9APM5 RZATAU
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.77 buffer MOPS, pH 6.5, apparent homogeneity Bilophila wadsworthia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.77 additional information
-
 activity depends strongly on growth substrate Bilophila wadsworthia
2.6.1.77 1.02
-
 taurine/formate-grown cells Bilophila wadsworthia
2.6.1.77 2.22
-
 pyruvate-grown cells Bilophila wadsworthia
2.6.1.77 35.3
-
 purified enzyme Bilophila wadsworthia

Storage Stability

EC Number Storage Stability Organism
2.6.1.77 4°C or 22°C, sulfonate buffers MES or MOPS, pH range 6.0-7.0, 22 h, stable Bilophila wadsworthia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.77 3-aminopropanesulfonate + pyruvate low activity Bilophila wadsworthia ?
-
 ?
2.6.1.77 3-aminopropanesulfonate + pyruvate purified enzyme Bilophila wadsworthia ?
-
 ?
2.6.1.77 beta-alanine + pyruvate purified enzyme Bilophila wadsworthia ?
-
 ?
2.6.1.77 cysteine + pyruvate low activity Bilophila wadsworthia ?
-
 ?
2.6.1.77 cysteine + pyruvate purified enzyme Bilophila wadsworthia ?
-
 ?
2.6.1.77 hypotaurine + pyruvate i.e. 2-aminoethanesulfinate Bilophila wadsworthia ?
-
 ?
2.6.1.77 hypotaurine + pyruvate purified enzyme Bilophila wadsworthia ?
-
 ?
2.6.1.77 hypotaurine + pyruvate i.e. 2-aminoethanesulfinate Bilophila wadsworthia RZATAU ?
-
 ?
2.6.1.77 hypotaurine + pyruvate purified enzyme Bilophila wadsworthia RZATAU ?
-
 ?
2.6.1.77 additional information no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor Bilophila wadsworthia ?
-
 ?
2.6.1.77 additional information cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates Bilophila wadsworthia ?
-
 ?
2.6.1.77 additional information no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor Bilophila wadsworthia RZATAU ?
-
 ?
2.6.1.77 additional information cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates Bilophila wadsworthia RZATAU ?
-
 ?
2.6.1.77 taurine + 2-oxobutyrate
-
 Bilophila wadsworthia 2-sulfoacetaldehyde + 2-aminobutyrate
-
 ?
2.6.1.77 taurine + oxaloacetate
-
 Bilophila wadsworthia ?
-
 ?
2.6.1.77 taurine + pyruvate i.e. 2-aminoethanesulfonate Bilophila wadsworthia 2-sulfoacetaldehyde + L-alanine
-
 r
2.6.1.77 taurine + pyruvate i.e. 2-aminoethanesulfonate Bilophila wadsworthia RZATAU 2-sulfoacetaldehyde + L-alanine
-
 r
2.6.1.77 taurine + pyruvate initial step in anaerobic taurine degradation Bilophila wadsworthia L-alanine + 2-sulfoacetaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.77 More formation of large aggregates depends on storage conditions, freezing at -20°C, or rapid freezing in liquid nitrogen with storage at -20°C increases the 350 kDa form, addition of glycerol decreases the aggregation, a dimer of 95 kDa is preferably formed during storage at 4°C Bilophila wadsworthia
2.6.1.77 tetramer 4 * 51000, SDS-PAGE Bilophila wadsworthia

Synonyms

EC Number Synonyms Comment Organism
2.6.1.77 tPA
-
 Bilophila wadsworthia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.77 35
-
-
 Bilophila wadsworthia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.77 20 40 broad optimum Bilophila wadsworthia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.77 9
-
-
 Bilophila wadsworthia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.77 additional information
-
 enzyme is unstable in buffers with neutral or alkaline pH value, except for HEPES at pH 7.0 Bilophila wadsworthia

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.77 pyridoxal 5'-phosphate activating Bilophila wadsworthia
2.6.1.77 pyridoxal 5'-phosphate pyridoxyl 5'-phosphate is not essentially required Bilophila wadsworthia