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Literature summary extracted from

  • Oda, T.; Miyajima, H.; Suzuki, Y.; Ito, T.; Yokota, S.; Hoshino, M.; Ichiyama, A.
    Purification and characterization of the active serine: pyruvate aminotransferase of rat liver mitochondria expressed in Escherichia coli (1989), J. Biochem., 106, 460-467.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.51 cDNA cloned and expressed in Escherichia coli DH1 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.51 0.0246
-
 glyoxylate pH 8.0, 30°C, native enzyme Rattus norvegicus
2.6.1.51 0.0336
-
 glyoxylate pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.6.1.51 1.1
-
 pyruvate pH 8.0, 30°C, native and recombinant enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.51 cytoplasm
-
 Rattus norvegicus 5737
-
2.6.1.51 mitochondrion
-
 Rattus norvegicus 5739
-
2.6.1.51 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.51 43000
-
 2 * 43000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.51 L-serine + pyruvate Rattus norvegicus
-
 3-hydroxypyruvate + L-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.51 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.51 native and recombinant enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.51 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.51 5.41
-
 recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.51 L-alanine + 3-hydroxypyruvate
-
 Rattus norvegicus pyruvate + L-serine
-
 r
2.6.1.51 L-serine + pyruvate
-
 Rattus norvegicus 3-hydroxypyruvate + L-alanine
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.51 dimer 2 * 43000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.51 pyridoxal 5'-phosphate
-
 Rattus norvegicus