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Literature summary extracted from

  • Ahlert, J.; Distler, J.; Mansouri, K.; Piepersberg, W.
    Identification of stsC, the gene encoding the L-glutamine:scyllo-inosose aminotransferase from streptomycin-producing Streptomycetes (1997), Arch. Microbiol., 168, 102-113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.50 gene StsC identified, cloned by PCR and expressed in Escherichia coli BL21 and Streptomyces lividans Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.50 45000
-
 2 * 45000, SDS-PAGE Streptomyces griseus
2.6.1.50 45500
-
 SDS-PAGE Streptomyces griseus
2.6.1.50 95000 100000 gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone Streptomyces bikiniensis
-
 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone Streptomyces hygroscopicus
-
 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone Streptomyces griseus streptidine subpathway of streptomycin biosynthesis 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone Streptomyces bikiniensis NRRL 1049b
-
 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.50 no activity in Escherichia coli
-
 host strain, free of enzymatic activity
-
2.6.1.50 no activity in Streptomyces lividans
-
 66 strain TK23, host strains, free of enzymatic activity
-
2.6.1.50 no activity in Streptomyces lividans
-
 66 strain 1326
-
2.6.1.50 Streptomyces bikiniensis
-
 NRRL 1049b
-
2.6.1.50 Streptomyces bikiniensis NRRL 1049b
-
 NRRL 1049b
-
2.6.1.50 Streptomyces griseus
-
 N2-3-11, DSM 40236
-
2.6.1.50 Streptomyces hygroscopicus
-
 subsp. glebosus DSM 40823
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.50 recombinant enzyme, pJAW76-2, expressed in Streptomyces lividans Streptomyces griseus

Storage Stability

EC Number Storage Stability Organism
2.6.1.50 6°C, purified enzyme stable for several weeks Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
-
 Streptomyces griseus 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
-
 Streptomyces bikiniensis 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
-
 Streptomyces hygroscopicus 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone streptidine subpathway of streptomycin biosynthesis Streptomyces griseus 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
-
 Streptomyces bikiniensis NRRL 1049b 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + scyllo-inosose
-
 Streptomyces griseus scyllo-inosamine + 2-oxoglutaramate spontaneously cyclization of 2-oxoglutaramate to 2-pyrrolidone-5-hydroxy-5-carboxylic acid r

Subunits

EC Number Subunits Comment Organism
2.6.1.50 dimer 2 * 45000, SDS-PAGE Streptomyces griseus