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Literature summary extracted from

  • Montemartini, M.; Santome, J.A.; Cazzulo, J.J.; Nowick, C.
    Purification and partial structural and kinetic characterization of tyrosine aminotransferase from epimastigotes of Trypanosoma cruzi (1993), Biochem. J., 292, 901-906.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.5
-
 pyruvate pH 7.0, 37°C, tyrosine as co-substrate Trypanosoma cruzi
2.6.1.5 6.8
-
 L-tyrosine pH 7.0, 37°C, pyruvate as co-substrate Trypanosoma cruzi
2.6.1.5 16
-
 oxaloacetate pH 7.0, 37°C, tyrosine as co-substrate Trypanosoma cruzi
2.6.1.5 17.9
-
 L-phenylalanine pH 7.0, 37°C, pyruvate as co-substrate Trypanosoma cruzi
2.6.1.5 21.4
-
 tryptophan pH 7.0, 37°C, pyruvate as co-substrate Trypanosoma cruzi
2.6.1.5 38
-
 2-oxoglutarate pH 7.0, 37°C, tyrosine as co-substrate Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.5 45000
-
 2 * 45000, SDS-PAGE Trypanosoma cruzi
2.6.1.5 91000
-
 gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Trypanosoma cruzi
-
 epimastigote
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.6.1.5 additional information not a glycoprotein Trypanosoma cruzi

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 partial, using chromatography on DEAE-cellulose and gel filtration Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.5 cell suspension culture
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.5 6.06
-
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-glutamate + oxaloacetate
-
 Trypanosoma cruzi L-aspartate + 2-oxoglutarate
-
 ?
2.6.1.5 L-glutamate + pyruvate
-
 Trypanosoma cruzi L-alanine + 2-oxoglutarate
-
 ?
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
 Trypanosoma cruzi L-glutamate + phenylpyruvate
-
 ?
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Trypanosoma cruzi 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.5 tryptophan + 2-oxoglutarate
-
 Trypanosoma cruzi 3-indole-2-oxopropanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.5 dimer 2 * 45000, SDS-PAGE Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.5 65
-
 complete inactivation after 5 min Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 7
-
-
 Trypanosoma cruzi