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Literature summary extracted from

  • Der Garabedian, P.A.
    Candida delta-aminovalerate: alpha-ketoglutarate aminotransferase: purification and enzymologic properties (1986), Biochemistry, 25, 5507-5512.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.48 2-oxoglutarate and pyridoxal 5'-phosphate protects against thermal denaturation Meyerozyma guilliermondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.48 2,3-Diaminopropionate noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 2,4-diaminobutyrate noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 alanine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 beta-Alanine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 glycine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 L-lysine noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 L-ornithine mixed inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 additional information alpha-aminobutyrate, 4-aminobutyrate, alpha-aminovalerate and epsilon-aminocaproate does not cause any inhibition Meyerozyma guilliermondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.48 3.6
-
 2-oxoglutarate pH 7.8, 35°C Meyerozyma guilliermondii
2.6.1.48 4.9
-
 delta-aminovaleric acid pH 7.8, 35°C Meyerozyma guilliermondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.48 60000
-
 2 * 60000, SDS-PAGE Meyerozyma guilliermondii
2.6.1.48 118000
-
 gel filtration Meyerozyma guilliermondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.48 5-aminopentanoate + 2-oxoglutarate Meyerozyma guilliermondii enzyme is strongly induced in cells grown on delta-aminovalerate or on lysine as only nitrogen source 5-oxovalerate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.48 Meyerozyma guilliermondii
-
 var.membranaefaciens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.48
-
 Meyerozyma guilliermondii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.48 20.8
-
-
 Meyerozyma guilliermondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.48 4-aminobutyrate + 2-oxoglutarate relative activity 40% Meyerozyma guilliermondii succinic semialdehyde + L-glutamate
-
 r
2.6.1.48 5-aminopentanoate + 2-oxoglutarate
-
 Meyerozyma guilliermondii 5-oxovalerate + L-glutamate
-
 r
2.6.1.48 5-aminopentanoate + 2-oxoglutarate enzyme is strongly induced in cells grown on delta-aminovalerate or on lysine as only nitrogen source Meyerozyma guilliermondii 5-oxovalerate + L-glutamate
-
 r
2.6.1.48 glycine + 2-oxoglutarate relative activity 1% Meyerozyma guilliermondii glyoxalate + L-glutamate
-
 r
2.6.1.48 L-leucine + 2-oxoglutarate relative activity 10% Meyerozyma guilliermondii 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.48 L-norleucine + 2-oxoglutarate relative activity 4.8% Meyerozyma guilliermondii 2-oxohexanoate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.48 dimer 2 * 60000, SDS-PAGE Meyerozyma guilliermondii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.48 40
-
-
 Meyerozyma guilliermondii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.48 50
-
 preserves 50% of its activity after being heated for 7 min, in presence of 2-oxoglutarate Meyerozyma guilliermondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.48 7.8 8.5
-
 Meyerozyma guilliermondii

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.48 pyridoxal 5'-phosphate Km 0.0227 mM Meyerozyma guilliermondii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.48 5
-
 beta-Alanine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 5.6
-
 L-ornithine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 7
-
 2,3-Diaminopropionate pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 9.5
-
 ornithine pH 7.8, 35°C, mixed inhibition Meyerozyma guilliermondii
2.6.1.48 10
-
 glycine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 11.5
-
 2,4-diaminobutyrate pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 12.5
-
 L-lysine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 15
-
 alanine pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate Meyerozyma guilliermondii
2.6.1.48 16
-
 beta-Alanine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
2.6.1.48 50
-
 2,3-Diaminopropionate pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
2.6.1.48 90
-
 glycine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
2.6.1.48 120
-
 alanine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
2.6.1.48 120
-
 lysine pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii
2.6.1.48 190
-
 2,4-diaminobutyrate pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate Meyerozyma guilliermondii