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Literature summary extracted from

  • Peters, P.; Galinski, E.A.; Trüper, H.G.
    The biosynthesis of ectoine (1990), FEMS Microbiol. Lett., 71, 157-162.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.46 1.2
-
 L-glutamate pH 9.1, 37ºC Halorhodospira halochloris
2.6.1.46 1.85
-
 L-glutamate
-
 Halomonas elongata
2.6.1.46 1.85
-
 L-glutamate pH 9.1, 37ºC Halorhodospira halochloris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.46 L-2-amino-4-oxobutanoate + L-glutamate Halorhodospira halochloris involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria L-2,4-diaminobutanoate + 2-oxoglutarate
-
 ?
2.6.1.46 L-2-amino-4-oxobutanoate + L-glutamate Halomonas elongata involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria L-2,4-diaminobutanoate + 2-oxoglutarate
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Halorhodospira halochloris final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
 r
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Halomonas elongata final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M L-ectoine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Halorhodospira halochloris
-
-
-
2.6.1.46 Halomonas elongata
-
 strain DSM 2581
-
2.6.1.46 Halorhodospira halochloris
-
 strain DSM 1059
-
4.2.1.108 Halomonas elongata
-
 extremely halophilic eubacterium, strain DSM 2581
-
4.2.1.108 Halorhodospira halochloris
-
 extremely halophilic eubacterium, strain DSM 1059
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.108 cell culture photosynthetically grown cells Halorhodospira halochloris
-
4.2.1.108 culture condition:glucose-grown cell
-
 Halomonas elongata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.178 0.114
-
-
 Halorhodospira halochloris
2.6.1.46 0.054
-
-
 Halorhodospira halochloris
2.6.1.46 0.171
-
-
 Halomonas elongata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Halorhodospira halochloris CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.6.1.46 L-2-amino-4-oxobutanoate + L-glutamate
-
 Halorhodospira halochloris L-2,4-diaminobutanoate + 2-oxoglutarate
-
 ?
2.6.1.46 L-2-amino-4-oxobutanoate + L-glutamate
-
 Halomonas elongata L-2,4-diaminobutanoate + 2-oxoglutarate
-
 ?
2.6.1.46 L-2-amino-4-oxobutanoate + L-glutamate involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria Halorhodospira halochloris L-2,4-diaminobutanoate + 2-oxoglutarate
-
 ?
2.6.1.46 L-2-amino-4-oxobutanoate + L-glutamate involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria Halomonas elongata L-2,4-diaminobutanoate + 2-oxoglutarate
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Halorhodospira halochloris L-ectoine + H2O
-
 r
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M Halomonas elongata L-ectoine + H2O
-
 r
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Halomonas elongata L-ectoine + H2O NMR and thin layer chromatography product identification r
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Halorhodospira halochloris L-ectoine + H2O NMR and TLC product identification r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.178 L-diaminobutyric acid acetyl transferase
-
 Halorhodospira halochloris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.178 35 40
-
 Halorhodospira halochloris
2.6.1.46 37
-
 assay at Halorhodospira halochloris
2.6.1.46 37
-
 assay at Halomonas elongata
4.2.1.108 37
-
 assay at Halorhodospira halochloris
4.2.1.108 37
-
 assay at Halomonas elongata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.178 8.2
-
-
 Halorhodospira halochloris
2.6.1.46 7.9
-
-
 Halomonas elongata
2.6.1.46 8.2
-
-
 Halorhodospira halochloris
4.2.1.108 9
-
 assay at Halorhodospira halochloris
4.2.1.108 9
-
 assay at Halomonas elongata