BRENDA - Enzyme Database show

L-alanine: 4,5-dioxovalerate transaminase in Leishmania donovani that differs from mammalian enzyme

Sagar, R.; Salotra, P.; Bhatnagar, R.; Datta, K.; Microbiol. Res. 150, 419-423 (1995)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.6.1.43
medicine
immunological difference between the enzyme from mammalian source and this enzyme presents a potential target for chemotherapy, development of treatment for Leishmaniasis, a major parasitic disease in humans and opportunistic infections in patients with AIDS, an inhibitor specific for the leishmanial enzyme may be used as a therapeutic agent
Leishmania donovani
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Leishmania donovani
2.6.1.43
methylglyoxal
-
Leishmania donovani
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
1.44
-
4,5-Dioxopentanoate
pH 7.0, 65°C
Leishmania donovani
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Leishmania donovani
first committed step in heme synthesis
5-aminolevulinate + pyruvate
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Leishmania donovani
-
AG83, WHO nomenclature MHOM/IN/83/AG83, promastigote form
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
beta-alanine + 4,5-dioxopentanoate
65.6% of the activity with L-alanine
640063
Leishmania donovani
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
81.2% of the activity with L-alanine
640063
Leishmania donovani
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640063
Leishmania donovani
5-aminolevulinate + pyruvate
-
640063
Leishmania donovani
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
first committed step in heme synthesis
640063
Leishmania donovani
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
43.8% of the activity with L-alanine
640063
Leishmania donovani
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-phenylalanine + 4,5-dioxopentanoate
90.6% of the activity with L-alanine
640063
Leishmania donovani
phenylpyruvate + 5-aminolevulinate
-
-
-
ir
2.6.1.43
additional information
cysteine is no substrate
640063
Leishmania donovani
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
65
-
-
Leishmania donovani
Application (protein specific)
EC Number
Application
Commentary
Organism
2.6.1.43
medicine
immunological difference between the enzyme from mammalian source and this enzyme presents a potential target for chemotherapy, development of treatment for Leishmaniasis, a major parasitic disease in humans and opportunistic infections in patients with AIDS, an inhibitor specific for the leishmanial enzyme may be used as a therapeutic agent
Leishmania donovani
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Leishmania donovani
2.6.1.43
methylglyoxal
-
Leishmania donovani
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
1.44
-
4,5-Dioxopentanoate
pH 7.0, 65°C
Leishmania donovani
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Leishmania donovani
first committed step in heme synthesis
5-aminolevulinate + pyruvate
-
-
ir
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
beta-alanine + 4,5-dioxopentanoate
65.6% of the activity with L-alanine
640063
Leishmania donovani
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
81.2% of the activity with L-alanine
640063
Leishmania donovani
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640063
Leishmania donovani
5-aminolevulinate + pyruvate
-
640063
Leishmania donovani
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
first committed step in heme synthesis
640063
Leishmania donovani
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
43.8% of the activity with L-alanine
640063
Leishmania donovani
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-phenylalanine + 4,5-dioxopentanoate
90.6% of the activity with L-alanine
640063
Leishmania donovani
phenylpyruvate + 5-aminolevulinate
-
-
-
ir
2.6.1.43
additional information
cysteine is no substrate
640063
Leishmania donovani
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
65
-
-
Leishmania donovani