BRENDA - Enzyme Database show

A rapid, high-yield purification of L-alanine:4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method

Tyagi, R.K.; Datta, K.; Protein Expr. Purif. 5, 527-533 (1994)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.43
repeated freezing and thawing causes inactivation
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
mitochondrion
mitochondrial matrix
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
50000
-
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
2.6.1.43
210000
-
gel filtration, gradient polyacrylamide gel electrophoresis
Rattus norvegicus
2.6.1.43
225000
-
gel filtration, Sepharose 6B
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Rattus norvegicus
-
Wistar
-
2.6.1.43
Rattus norvegicus Wistar
-
Wistar
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
kidney
-
Rattus norvegicus
-
2.6.1.43
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
36.9
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
-20°C, can be stored for nearly 1 year without significant loss of enzyme activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
tetramer
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
60
-
-
Rattus norvegicus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.6.1.43
20
80
3fold increase of activity from 37°C to 60°C
Rattus norvegicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.43
55
65
retains more than 90% maximum activity through 55-65°C, beyond 65°C activity decreases sharply
Rattus norvegicus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.6.1.43
Rattus norvegicus
isoelectric focusing analysis, 2-dimensional gel electrophoresis
-
5
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.43
repeated freezing and thawing causes inactivation
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
mitochondrion
mitochondrial matrix
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
50000
-
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
2.6.1.43
210000
-
gel filtration, gradient polyacrylamide gel electrophoresis
Rattus norvegicus
2.6.1.43
225000
-
gel filtration, Sepharose 6B
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
kidney
-
Rattus norvegicus
-
2.6.1.43
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
36.9
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
-20°C, can be stored for nearly 1 year without significant loss of enzyme activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
tetramer
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
60
-
-
Rattus norvegicus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.6.1.43
20
80
3fold increase of activity from 37°C to 60°C
Rattus norvegicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.43
55
65
retains more than 90% maximum activity through 55-65°C, beyond 65°C activity decreases sharply
Rattus norvegicus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.6.1.43
Rattus norvegicus
isoelectric focusing analysis, 2-dimensional gel electrophoresis
-
5