Literature summary extracted from

Tyagi, R.K.; Datta, K.
A rapid, high-yield purification of L-alanine:4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method (1994), Protein Expr. Purif., 5, 527-533.

General Stability

EC Number	General Stability	Organism
2.6.1.43	repeated freezing and thawing causes inactivation	Rattus norvegicus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.6.1.43	hemin	-	Rattus norvegicus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.6.1.43	mitochondrion	mitochondrial matrix	Rattus norvegicus	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.6.1.43	50000	-	4 * 50000, mitochondrial isozyme, SDS-PAGE	Rattus norvegicus
2.6.1.43	210000	-	gel filtration, gradient polyacrylamide gel electrophoresis	Rattus norvegicus
2.6.1.43	225000	-	gel filtration, Sepharose 6B	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.6.1.43	L-alanine + 4,5-dioxopentanoate	Rattus norvegicus	alternate route for delta-aminolevulinate formation	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	Rattus norvegicus	heme biosynthetic pathway	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	Rattus norvegicus Wistar	alternate route for delta-aminolevulinate formation	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	Rattus norvegicus Wistar	heme biosynthetic pathway	5-aminolevulinate + pyruvate	-	ir

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.6.1.43	Rattus norvegicus	-	Wistar	-
2.6.1.43	Rattus norvegicus Wistar	-	Wistar	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.6.1.43	-	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.6.1.43	kidney	-	Rattus norvegicus	-
2.6.1.43	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.6.1.43	36.9	-	-	Rattus norvegicus

Storage Stability

EC Number	Storage Stability	Organism
2.6.1.43	-20°C, can be stored for nearly 1 year without significant loss of enzyme activity	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.6.1.43	L-alanine + 4,5-dioxopentanoate	-	Rattus norvegicus	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	alternate route for delta-aminolevulinate formation	Rattus norvegicus	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	heme biosynthetic pathway	Rattus norvegicus	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	-	Rattus norvegicus Wistar	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	alternate route for delta-aminolevulinate formation	Rattus norvegicus Wistar	5-aminolevulinate + pyruvate	-	ir
2.6.1.43	L-alanine + 4,5-dioxopentanoate	heme biosynthetic pathway	Rattus norvegicus Wistar	5-aminolevulinate + pyruvate	-	ir

Subunits

EC Number	Subunits	Comment	Organism
2.6.1.43	tetramer	4 * 50000, mitochondrial isozyme, SDS-PAGE	Rattus norvegicus

Synonyms

EC Number	Synonyms	Comment	Organism
2.6.1.43	EC 2.6.1.19	2-aminobutyrate aminotransferase and aminolevulinate transaminase activity reside in the same protein	Rattus norvegicus
2.6.1.43	EC 2.6.1.40	dimethylarginine-pyruvate aminotransferase and D-3-aminoisobutyrate pyruvate aminotransferase activities resides in the same protein	Rattus norvegicus
2.6.1.43	EC 2.6.1.44	alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein	Rattus norvegicus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.6.1.43	60	-	-	Rattus norvegicus

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
2.6.1.43	20	80	3fold increase of activity from 37°C to 60°C	Rattus norvegicus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.6.1.43	55	65	retains more than 90% maximum activity through 55-65°C, beyond 65°C activity decreases sharply	Rattus norvegicus

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
2.6.1.43	Rattus norvegicus	isoelectric focusing analysis, 2-dimensional gel electrophoresis	-	5

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Release 2023.1 (January 2023)