BRENDA - Enzyme Database show

Characteristics of L-alanine:4,5-dioxovaleric acid transaminase: an alternate pathway of heme biosynthesis in yeast

Hoare, K.; Datta, K.; Arch. Biochem. Biophys. 277, 122-129 (1990)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.43
repeated freezing and thawing inactivates the enzyme, stability of the enzyme is enhanced by presence of 5% glycerol
Candida albicans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
additional information
EDTA, N-ethylmaleimide. iodoacetic acid, cysteine and beta-mercaptoethanol are no inhibitors
Candida albicans
2.6.1.43
p-chloromercuribenzoate
-
Candida albicans
2.6.1.43
Tris-HCl
-
Candida albicans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.00028
-
L-alanine
pH 7.2, 60°C
Candida albicans
2.6.1.43
0.0105
-
4,5-Dioxopentanoate
pH 7.2, 60°C
Candida albicans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
cytosol
-
Candida albicans
5829
-
2.6.1.43
mitochondrion
-
Candida albicans
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
57544
-
1 * 57544, SDS-PAGE
Candida albicans
2.6.1.43
59000
-
gel filtration
Candida albicans
2.6.1.43
59000
-
gel filtration
Saccharomyces cerevisiae
2.6.1.43
60260
-
gel filtration
Candida albicans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Saccharomyces cerevisiae
-
5-aminolevulinate + pyruvate
-
Saccharomyces cerevisiae
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Candida albicans
-
5-aminolevulinate + pyruvate
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Candida albicans 3100
-
5-aminolevulinate + pyruvate
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Saccharomyces cerevisiae 3059 S288C
-
5-aminolevulinate + pyruvate
-
Saccharomyces cerevisiae 3059 S288C
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Candida albicans
-
3100
-
2.6.1.43
Candida albicans 3100
-
3100
-
2.6.1.43
Saccharomyces cerevisiae
-
3059, S288C
-
2.6.1.43
Saccharomyces cerevisiae 3059 S288C
-
3059, S288C
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Candida albicans
2.6.1.43
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
0.186
-
-
Candida albicans
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
4°C, stored in ice for 3 months without any appreciable loss of enzyme activity
Candida albicans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
glycine + 4,5-dioxopentanoate
-
640057
Candida albicans
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
-
640057
Candida albicans 3100
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Saccharomyces cerevisiae
5-aminolevulinate + pyruvate
-
640057
Saccharomyces cerevisiae
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans
5-aminolevulinate + pyruvate
-
640057
Candida albicans
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans 3100
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans 3100
5-aminolevulinate + pyruvate
-
640057
Candida albicans 3100
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Saccharomyces cerevisiae 3059 S288C
5-aminolevulinate + pyruvate
-
640057
Saccharomyces cerevisiae 3059 S288C
ir
2.6.1.43
L-arginine + 4,5-dioxopentanoate
6.8% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 5-guanidino-2-oxopentanoate
-
-
-
ir
2.6.1.43
L-asparagine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 4-amino-2,4-dioxobutanoic acid
-
-
-
ir
2.6.1.43
L-aspartate + 4,5-dioxopentanoate
39% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + oxaloacetate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
39% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
39% of the activity with L-alanine
640057
Candida albicans 3100
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 2-oxoglutaramate
-
-
-
-
2.6.1.43
additional information
cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors
640057
Candida albicans
?
-
-
-
-
2.6.1.43
additional information
cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors
640057
Candida albicans 3100
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
monomer
1 * 57544, SDS-PAGE
Candida albicans
2.6.1.43
monomer
1 * 59000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
60
-
-
Candida albicans
2.6.1.43
60
-
-
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
7.2
-
-
Candida albicans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
Km 0.0005 mM
Candida albicans
2.6.1.43
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.6.1.43
Candida albicans
isoelectric focusing using Phast GEl IEF 3-9
4.8
4.7
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
Km 0.0005 mM
Candida albicans
2.6.1.43
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.43
repeated freezing and thawing inactivates the enzyme, stability of the enzyme is enhanced by presence of 5% glycerol
Candida albicans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
additional information
EDTA, N-ethylmaleimide. iodoacetic acid, cysteine and beta-mercaptoethanol are no inhibitors
Candida albicans
2.6.1.43
p-chloromercuribenzoate
-
Candida albicans
2.6.1.43
Tris-HCl
-
Candida albicans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.00028
-
L-alanine
pH 7.2, 60°C
Candida albicans
2.6.1.43
0.0105
-
4,5-Dioxopentanoate
pH 7.2, 60°C
Candida albicans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
cytosol
-
Candida albicans
5829
-
2.6.1.43
mitochondrion
-
Candida albicans
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
57544
-
1 * 57544, SDS-PAGE
Candida albicans
2.6.1.43
59000
-
gel filtration
Candida albicans
2.6.1.43
59000
-
gel filtration
Saccharomyces cerevisiae
2.6.1.43
60260
-
gel filtration
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Saccharomyces cerevisiae
-
5-aminolevulinate + pyruvate
-
Saccharomyces cerevisiae
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Candida albicans
-
5-aminolevulinate + pyruvate
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Candida albicans 3100
-
5-aminolevulinate + pyruvate
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Saccharomyces cerevisiae 3059 S288C
-
5-aminolevulinate + pyruvate
-
Saccharomyces cerevisiae 3059 S288C
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Candida albicans
2.6.1.43
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
0.186
-
-
Candida albicans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
4°C, stored in ice for 3 months without any appreciable loss of enzyme activity
Candida albicans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
glycine + 4,5-dioxopentanoate
-
640057
Candida albicans
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
-
640057
Candida albicans 3100
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Saccharomyces cerevisiae
5-aminolevulinate + pyruvate
-
640057
Saccharomyces cerevisiae
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans
5-aminolevulinate + pyruvate
-
640057
Candida albicans
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans 3100
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Candida albicans 3100
5-aminolevulinate + pyruvate
-
640057
Candida albicans 3100
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640057
Saccharomyces cerevisiae 3059 S288C
5-aminolevulinate + pyruvate
-
640057
Saccharomyces cerevisiae 3059 S288C
ir
2.6.1.43
L-arginine + 4,5-dioxopentanoate
6.8% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 5-guanidino-2-oxopentanoate
-
-
-
ir
2.6.1.43
L-asparagine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 4-amino-2,4-dioxobutanoic acid
-
-
-
ir
2.6.1.43
L-aspartate + 4,5-dioxopentanoate
39% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + oxaloacetate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
39% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
39% of the activity with L-alanine
640057
Candida albicans 3100
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640057
Candida albicans
5-aminolevulinate + 2-oxoglutaramate
-
-
-
-
2.6.1.43
additional information
cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors
640057
Candida albicans
?
-
-
-
-
2.6.1.43
additional information
cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors
640057
Candida albicans 3100
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
monomer
1 * 57544, SDS-PAGE
Candida albicans
2.6.1.43
monomer
1 * 59000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
60
-
-
Candida albicans
2.6.1.43
60
-
-
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
7.2
-
-
Candida albicans
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.6.1.43
Candida albicans
isoelectric focusing using Phast GEl IEF 3-9
4.8
4.7