BRENDA - Enzyme Database show

L-alanine: 4,5-dioxovalerate aminotransferase from Pseudomonas riboflavina: purification and inactivation by methylglyoxal

Rhee, H.i.; Murata, K.; Kimura, A.; J. Biochem. 103, 1045-1049 (1988)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.6.1.43
crystallized as bright yellow needles
Devosia riboflavina
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Devosia riboflavina
2.6.1.43
methylglyoxal
-
Devosia riboflavina
2.6.1.43
pyruvate
-
Devosia riboflavina
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.75
-
4,5-Dioxopentanoate
pH 8.0, 37░C
Devosia riboflavina
2.6.1.43
1.7
-
L-alanine
pH 8.0, 37░C
Devosia riboflavina
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
48000
-
4 * 48000, SDS-PAGE
Devosia riboflavina
2.6.1.43
190000
-
gel filtration
Devosia riboflavina
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Devosia riboflavina
delta-aminolevulinate pathway
5-aminolevulinate + pyruvate
-
Devosia riboflavina
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Devosia riboflavina
-
IFO 3140
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Devosia riboflavina
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6.1.43
1.02
-
-
Devosia riboflavina
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
-15░C, stable for 3 months
Devosia riboflavina
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
beta-alanine + 4,5-dioxopentanoate
39% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
2.6.1.43
epsilon-amino-n-caproate + 4,5-dioxopentanoate
34% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
2.6.1.43
gamma-amino-n-butyrate + 4,5-dioxopentanoate
55% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
delta-aminolevulinate pathway
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
2.6.1.43
L-ornithine + 4,5-dioxopentanoate
14% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
2.6.1.43
additional information
L-lysine, L-arginine, L-glutamate, L-methionine, L-histidine, glycine, L-phenylalanine, L-threonine, L-asparagine, L-serine, L-proline, L-valine, L-isoleucine, L-leucine, L-tyrosine, L-cysteine and L-tryptophan are all inert as amino donors, methylglyoxal is a poor substrate for the enzyme
640054
Devosia riboflavina
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
tetramer
4 * 48000, SDS-PAGE
Devosia riboflavina
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Devosia riboflavina
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Devosia riboflavina
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.6.1.43
crystallized as bright yellow needles
Devosia riboflavina
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Devosia riboflavina
2.6.1.43
methylglyoxal
-
Devosia riboflavina
2.6.1.43
pyruvate
-
Devosia riboflavina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.75
-
4,5-Dioxopentanoate
pH 8.0, 37░C
Devosia riboflavina
2.6.1.43
1.7
-
L-alanine
pH 8.0, 37░C
Devosia riboflavina
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
48000
-
4 * 48000, SDS-PAGE
Devosia riboflavina
2.6.1.43
190000
-
gel filtration
Devosia riboflavina
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Devosia riboflavina
delta-aminolevulinate pathway
5-aminolevulinate + pyruvate
-
Devosia riboflavina
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Devosia riboflavina
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6.1.43
1.02
-
-
Devosia riboflavina
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
-15░C, stable for 3 months
Devosia riboflavina
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
beta-alanine + 4,5-dioxopentanoate
39% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
2.6.1.43
epsilon-amino-n-caproate + 4,5-dioxopentanoate
34% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
2.6.1.43
gamma-amino-n-butyrate + 4,5-dioxopentanoate
55% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
delta-aminolevulinate pathway
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
2.6.1.43
L-ornithine + 4,5-dioxopentanoate
14% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
2.6.1.43
additional information
L-lysine, L-arginine, L-glutamate, L-methionine, L-histidine, glycine, L-phenylalanine, L-threonine, L-asparagine, L-serine, L-proline, L-valine, L-isoleucine, L-leucine, L-tyrosine, L-cysteine and L-tryptophan are all inert as amino donors, methylglyoxal is a poor substrate for the enzyme
640054
Devosia riboflavina
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
tetramer
4 * 48000, SDS-PAGE
Devosia riboflavina