EC Number | General Stability | Organism |
---|---|---|
2.6.1.43 | purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.43 | 2-oxoglutarate | - |
Rattus norvegicus | |
2.6.1.43 | 4,5-Dioxopentanoate | - |
Rattus norvegicus | |
2.6.1.43 | hemin | uncompetitive inhibitor | Rattus norvegicus | |
2.6.1.43 | iodoacetate | - |
Rattus norvegicus | |
2.6.1.43 | methylglyoxal | - |
Rattus norvegicus | |
2.6.1.43 | p-chloromercuric benzene sulfonate | - |
Rattus norvegicus | |
2.6.1.43 | pyruvate | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.43 | 0.28 | - |
4,5-Dioxopentanoate | pH 6.9, 37°C | Rattus norvegicus | |
2.6.1.43 | 3.3 | - |
L-alanine | pH 6.9, 37°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.43 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.43 | 41000 | - |
6 * 41000 | Rattus norvegicus |
2.6.1.43 | 232000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.43 | L-alanine + 4,5-dioxopentanoate | Rattus norvegicus | - |
5-aminolevulinate + pyruvate | - |
ir | |
2.6.1.43 | L-alanine + 4,5-dioxopentanoate | Rattus norvegicus Wistar | - |
5-aminolevulinate + pyruvate | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.43 | Rattus norvegicus | - |
Wistar | - |
2.6.1.43 | Rattus norvegicus Wistar | - |
Wistar | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.43 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.43 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.43 | 1.171 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.43 | -20°C, remains stable for at least 1 month | Rattus norvegicus |
2.6.1.43 | -70°C, stable for at least 2-3 months | Rattus norvegicus |
2.6.1.43 | 4°C, can be stored for at least 1 week without any significant loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.43 | L-alanine + 4,5-dioxopentanoate | - |
Rattus norvegicus | 5-aminolevulinate + pyruvate | - |
ir | |
2.6.1.43 | L-alanine + 4,5-dioxopentanoate | - |
Rattus norvegicus Wistar | 5-aminolevulinate + pyruvate | - |
ir | |
2.6.1.43 | additional information | DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine | Rattus norvegicus | ? | - |
? | |
2.6.1.43 | additional information | DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine | Rattus norvegicus Wistar | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.43 | hexamer | 6 * 41000 | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.43 | pyridoxal 5'-phosphate | - |
Rattus norvegicus |