BRENDA - Enzyme Database show

Affinity purification and properties of rat liver mitochondrial L-alanine:4,5-dioxovalerate transaminase and its inhibition by hemin

Shanker, J.; Datta, K.; Arch. Biochem. Biophys. 248, 652-657 (1986)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.43
purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Rattus norvegicus
2.6.1.43
4,5-Dioxopentanoate
-
Rattus norvegicus
2.6.1.43
hemin
uncompetitive inhibitor
Rattus norvegicus
2.6.1.43
iodoacetate
-
Rattus norvegicus
2.6.1.43
methylglyoxal
-
Rattus norvegicus
2.6.1.43
p-chloromercuric benzene sulfonate
-
Rattus norvegicus
2.6.1.43
pyruvate
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.28
-
4,5-Dioxopentanoate
pH 6.9, 37°C
Rattus norvegicus
2.6.1.43
3.3
-
L-alanine
pH 6.9, 37°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
41000
-
6 * 41000
Rattus norvegicus
2.6.1.43
232000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
-
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
-
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Rattus norvegicus
-
Wistar
-
2.6.1.43
Rattus norvegicus Wistar
-
Wistar
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
1.171
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
-20°C, remains stable for at least 1 month
Rattus norvegicus
2.6.1.43
-70°C, stable for at least 2-3 months
Rattus norvegicus
2.6.1.43
4°C, can be stored for at least 1 week without any significant loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640053
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640053
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640053
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640053
Rattus norvegicus Wistar
ir
2.6.1.43
additional information
DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine
640053
Rattus norvegicus
?
-
-
-
-
2.6.1.43
additional information
DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine
640053
Rattus norvegicus Wistar
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
hexamer
6 * 41000
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.43
purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Rattus norvegicus
2.6.1.43
4,5-Dioxopentanoate
-
Rattus norvegicus
2.6.1.43
hemin
uncompetitive inhibitor
Rattus norvegicus
2.6.1.43
iodoacetate
-
Rattus norvegicus
2.6.1.43
methylglyoxal
-
Rattus norvegicus
2.6.1.43
p-chloromercuric benzene sulfonate
-
Rattus norvegicus
2.6.1.43
pyruvate
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.28
-
4,5-Dioxopentanoate
pH 6.9, 37°C
Rattus norvegicus
2.6.1.43
3.3
-
L-alanine
pH 6.9, 37°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
41000
-
6 * 41000
Rattus norvegicus
2.6.1.43
232000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
-
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
-
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
1.171
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
-20°C, remains stable for at least 1 month
Rattus norvegicus
2.6.1.43
-70°C, stable for at least 2-3 months
Rattus norvegicus
2.6.1.43
4°C, can be stored for at least 1 week without any significant loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640053
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640053
Rattus norvegicus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640053
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640053
Rattus norvegicus Wistar
ir
2.6.1.43
additional information
DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine
640053
Rattus norvegicus
?
-
-
-
-
2.6.1.43
additional information
DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine
640053
Rattus norvegicus Wistar
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
hexamer
6 * 41000
Rattus norvegicus