BRENDA - Enzyme Database show

Purification and characterization of rat kidney L-alanine: 4,5-dioxovalerate transaminase and inhibition by hemin

Singh, N.K.; Datta, K.; Biochim. Biophys. Acta 827, 305-309 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Rattus norvegicus
2.6.1.43
protoporphyrin
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.25
-
4,5-Dioxopentanoate
pH 7.6, 37°C
Rattus norvegicus
2.6.1.43
2.9
-
L-alanine
pH 7.6, 37°C
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
37000
-
6 * 37000, SDS-PAGE
Rattus norvegicus
2.6.1.43
225000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
-
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
1.05
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640052
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640052
Rattus norvegicus
ir
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
hexamer
6 * 37000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
65
-
-
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
6.6
-
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
hemin
-
Rattus norvegicus
2.6.1.43
protoporphyrin
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.25
-
4,5-Dioxopentanoate
pH 7.6, 37°C
Rattus norvegicus
2.6.1.43
2.9
-
L-alanine
pH 7.6, 37°C
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
37000
-
6 * 37000, SDS-PAGE
Rattus norvegicus
2.6.1.43
225000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
-
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
1.05
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640052
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640052
Rattus norvegicus
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
hexamer
6 * 37000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.43
65
-
-
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
6.6
-
-
Rattus norvegicus