BRENDA - Enzyme Database show

delta-Aminolevulinic acid formation. Purification and properties of alanine:4,5-dioxovalerate, aminotransferase and isolation of 4,5-dioxovalerate from Clostridium tetanomorphum

Bajkowski, A.S.; Friedmann, H.C.; J. Biol. Chem. 257, 2207-2211 (1982)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Clostridium tetanomorphum
2.6.1.43
amino-oxyacetic acid
-
Clostridium tetanomorphum
2.6.1.43
diacetyl
-
Clostridium tetanomorphum
2.6.1.43
Glyoxal
-
Clostridium tetanomorphum
2.6.1.43
glyoxylic acid
-
Clostridium tetanomorphum
2.6.1.43
methylglyoxal
-
Clostridium tetanomorphum
2.6.1.43
oxaloacetic acid
-
Clostridium tetanomorphum
2.6.1.43
pyruvate
-
Clostridium tetanomorphum
2.6.1.43
succinate semialdehyde
-
Clostridium tetanomorphum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.26
-
4,5-Dioxopentanoate
pH 6.9, 37░C
Clostridium tetanomorphum
2.6.1.43
1.96
-
L-alanine
pH 6.9, 37░C
Clostridium tetanomorphum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
55500
-
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
2.6.1.43
72000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer containing 4 M NaCl
Clostridium tetanomorphum
2.6.1.43
78000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer
Clostridium tetanomorphum
2.6.1.43
82000
-
gel filtration
Clostridium tetanomorphum
2.6.1.43
111000
-
gel filtration
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Clostridium tetanomorphum
-
5-aminolevulinate + pyruvate
-
Clostridium tetanomorphum
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Clostridium tetanomorphum
-
ATCC 15920
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6.1.43
7.68
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640048
Clostridium tetanomorphum
5-aminolevulinate + pyruvate
-
640048
Clostridium tetanomorphum
ir
2.6.1.43
L-aspartate + 4,5-dioxopentanoate
67% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + oxaloacetate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
96% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-histidine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
-
-
ir
2.6.1.43
L-phenylalanine + 4,5-dioxopentanoate
71% of the activity with L-alanine
640048
Clostridium tetanomorphum
phenylpyruvate + 5-aminolevulinate
-
-
-
ir
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
dimer
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Clostridium tetanomorphum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Clostridium tetanomorphum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Clostridium tetanomorphum
2.6.1.43
amino-oxyacetic acid
-
Clostridium tetanomorphum
2.6.1.43
diacetyl
-
Clostridium tetanomorphum
2.6.1.43
Glyoxal
-
Clostridium tetanomorphum
2.6.1.43
glyoxylic acid
-
Clostridium tetanomorphum
2.6.1.43
methylglyoxal
-
Clostridium tetanomorphum
2.6.1.43
oxaloacetic acid
-
Clostridium tetanomorphum
2.6.1.43
pyruvate
-
Clostridium tetanomorphum
2.6.1.43
succinate semialdehyde
-
Clostridium tetanomorphum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.26
-
4,5-Dioxopentanoate
pH 6.9, 37░C
Clostridium tetanomorphum
2.6.1.43
1.96
-
L-alanine
pH 6.9, 37░C
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
55500
-
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
2.6.1.43
72000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer containing 4 M NaCl
Clostridium tetanomorphum
2.6.1.43
78000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer
Clostridium tetanomorphum
2.6.1.43
82000
-
gel filtration
Clostridium tetanomorphum
2.6.1.43
111000
-
gel filtration
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Clostridium tetanomorphum
-
5-aminolevulinate + pyruvate
-
Clostridium tetanomorphum
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Clostridium tetanomorphum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6.1.43
7.68
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640048
Clostridium tetanomorphum
5-aminolevulinate + pyruvate
-
640048
Clostridium tetanomorphum
ir
2.6.1.43
L-aspartate + 4,5-dioxopentanoate
67% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + oxaloacetate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
96% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-histidine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
-
-
ir
2.6.1.43
L-phenylalanine + 4,5-dioxopentanoate
71% of the activity with L-alanine
640048
Clostridium tetanomorphum
phenylpyruvate + 5-aminolevulinate
-
-
-
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
dimer
2 * 55500, SDS-PAGE
Clostridium tetanomorphum