BRENDA - Enzyme Database show

Biosynthesis of porphyrin precursors in mammals. Identity of alanine: gamma, delta-dioxovalerate aminotransferase with alanine:glyoxylate aminotransferase

Noguchi, T.; Mori, R.; J. Biol. Chem. 256, 10335-10339 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
glyoxylic acid
competitive inhibition
Bos taurus
2.6.1.43
hydroxylamine
-
Bos taurus
2.6.1.43
Isonicotinic acid hydrazide
-
Bos taurus
2.6.1.43
potassium cyanide
-
Bos taurus
2.6.1.43
Semicarbazide
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.3
-
4,5-Dioxopentanoate
pH 7.0, 37C
Bos taurus
2.6.1.43
0.67
-
glyoxylate
pH 7.0, 37C
Bos taurus
2.6.1.43
2.5
-
L-alanine
pH 7.0, 37C, 4,5-dioxopentanoate as amino acceptor
Bos taurus
2.6.1.43
4
-
L-alanine
pH 7.0, 37C, glyoxylate as amino acceptor
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
58000
-
4 * 58000, SDS-PAGE
Bos taurus
2.6.1.43
238000
-
gel filtration
Bos taurus
2.6.1.43
240000
-
-
Bos taurus
2.6.1.43
245000
-
sucrose density gradient centrifugation
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Bos taurus
biosynthesis of porphyrin precursors
5-aminolevulinate + pyruvate
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Bos taurus
-
bovine
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.43
0.753
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
-20C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, may be stored for at least 8 weeks without loss of either activity
Bos taurus
2.6.1.43
0-5C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, stored for 2 weeks, little or none of each activity is lost
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
glycine + 4,5-dioxopentanoate
-
640047
Bos taurus
5-aminolevulinate + glyoxylate
-
-
-
-
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640047
Bos taurus
5-aminolevulinate + pyruvate
-
640047
Bos taurus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
biosynthesis of porphyrin precursors
640047
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
tetramer
4 * 58000, SDS-PAGE
Bos taurus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.43
60
-
10 min, about 65% loss of activity
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
glyoxylic acid
competitive inhibition
Bos taurus
2.6.1.43
hydroxylamine
-
Bos taurus
2.6.1.43
Isonicotinic acid hydrazide
-
Bos taurus
2.6.1.43
potassium cyanide
-
Bos taurus
2.6.1.43
Semicarbazide
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.3
-
4,5-Dioxopentanoate
pH 7.0, 37C
Bos taurus
2.6.1.43
0.67
-
glyoxylate
pH 7.0, 37C
Bos taurus
2.6.1.43
2.5
-
L-alanine
pH 7.0, 37C, 4,5-dioxopentanoate as amino acceptor
Bos taurus
2.6.1.43
4
-
L-alanine
pH 7.0, 37C, glyoxylate as amino acceptor
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
58000
-
4 * 58000, SDS-PAGE
Bos taurus
2.6.1.43
238000
-
gel filtration
Bos taurus
2.6.1.43
240000
-
-
Bos taurus
2.6.1.43
245000
-
sucrose density gradient centrifugation
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Bos taurus
biosynthesis of porphyrin precursors
5-aminolevulinate + pyruvate
-
-
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.43
0.753
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
-20C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, may be stored for at least 8 weeks without loss of either activity
Bos taurus
2.6.1.43
0-5C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, stored for 2 weeks, little or none of each activity is lost
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
glycine + 4,5-dioxopentanoate
-
640047
Bos taurus
5-aminolevulinate + glyoxylate
-
-
-
-
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640047
Bos taurus
5-aminolevulinate + pyruvate
-
640047
Bos taurus
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
biosynthesis of porphyrin precursors
640047
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
tetramer
4 * 58000, SDS-PAGE
Bos taurus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.43
60
-
10 min, about 65% loss of activity
Bos taurus