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Literature summary extracted from

  • Noguchi, T.; Mori, R.
    Biosynthesis of porphyrin precursors in mammals. Identity of alanine: gamma, delta-dioxovalerate aminotransferase with alanine:glyoxylate aminotransferase (1981), J. Biol. Chem., 256, 10335-10339.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.43 glyoxylic acid competitive inhibition Bos taurus
2.6.1.43 hydroxylamine
-
 Bos taurus
2.6.1.43 Isonicotinic acid hydrazide
-
 Bos taurus
2.6.1.43 potassium cyanide
-
 Bos taurus
2.6.1.43 Semicarbazide
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.43 0.3
-
 4,5-Dioxopentanoate pH 7.0, 37°C Bos taurus
2.6.1.43 0.67
-
 glyoxylate pH 7.0, 37°C Bos taurus
2.6.1.43 2.5
-
 L-alanine pH 7.0, 37°C, 4,5-dioxopentanoate as amino acceptor Bos taurus
2.6.1.43 4
-
 L-alanine pH 7.0, 37°C, glyoxylate as amino acceptor Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.43 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.43 58000
-
 4 * 58000, SDS-PAGE Bos taurus
2.6.1.43 238000
-
 gel filtration Bos taurus
2.6.1.43 240000
-
-
 Bos taurus
2.6.1.43 245000
-
 sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate Bos taurus biosynthesis of porphyrin precursors 5-aminolevulinate + pyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.43 Bos taurus
-
 bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.43
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.43 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.43 0.753
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.6.1.43 -20°C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, may be stored for at least 8 weeks without loss of either activity Bos taurus
2.6.1.43 0-5°C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, stored for 2 weeks, little or none of each activity is lost Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.43 glycine + 4,5-dioxopentanoate
-
 Bos taurus 5-aminolevulinate + glyoxylate
-
 ?
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Bos taurus 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate biosynthesis of porphyrin precursors Bos taurus 5-aminolevulinate + pyruvate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.6.1.43 tetramer 4 * 58000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.43 EC 2.6.1.44 alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein Bos taurus
2.6.1.43 EC 2.6.1.44 identical with alanine-glyoxylate aminotransferase Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.43 60
-
 10 min, about 65% loss of activity Bos taurus