BRENDA - Enzyme Database show

gamma, delta-Dioxovalerate aminotransferase activity in Rhodopseudomonas spheroides

Neuberger, A.; Turner, J.M.; Biochim. Biophys. Acta 67, 342-345 (1963)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.43
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM
Rhodobacter sphaeroides
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
4,5-Dioxopentanoate
substrate inhibition
Rhodobacter sphaeroides
2.6.1.43
acetyl acetone
-
Rhodobacter sphaeroides
2.6.1.43
amino-oxyacetic acid
-
Rhodobacter sphaeroides
2.6.1.43
benzoyl acetone
-
Rhodobacter sphaeroides
2.6.1.43
beta-Alanine
8% inhibition
Rhodobacter sphaeroides
2.6.1.43
beta-Iodopropionate
30% inhibition
Rhodobacter sphaeroides
2.6.1.43
cadaverine
14% inhibition
Rhodobacter sphaeroides
2.6.1.43
cysteamine
-
Rhodobacter sphaeroides
2.6.1.43
cysteine
90% inhibition
Rhodobacter sphaeroides
2.6.1.43
diacetyl
-
Rhodobacter sphaeroides
2.6.1.43
ethyl acetoacetate
-
Rhodobacter sphaeroides
2.6.1.43
glyoxylic acid
-
Rhodobacter sphaeroides
2.6.1.43
homocysteine
-
Rhodobacter sphaeroides
2.6.1.43
iodoacetate
30% inhibition
Rhodobacter sphaeroides
2.6.1.43
Isonicotinic acid hydrazide
-
Rhodobacter sphaeroides
2.6.1.43
lysine
20% inhibition
Rhodobacter sphaeroides
2.6.1.43
additional information
iodoacetamide and chloroacetamide are not inhibitors
Rhodobacter sphaeroides
2.6.1.43
ophthalaldehyde
-
Rhodobacter sphaeroides
2.6.1.43
ornithine
20% inhibition
Rhodobacter sphaeroides
2.6.1.43
oxaloacetic acid
-
Rhodobacter sphaeroides
2.6.1.43
p-chloromercuribenzoate
78% inhibition
Rhodobacter sphaeroides
2.6.1.43
Penicillamine
-
Rhodobacter sphaeroides
2.6.1.43
Pyruvic acid
-
Rhodobacter sphaeroides
2.6.1.43
succinic acid
-
Rhodobacter sphaeroides
2.6.1.43
Thioglycollate
74% inhibition
Rhodobacter sphaeroides
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.4
-
4,5-Dioxopentanoate
pH 7.0, 37°C
Rhodobacter sphaeroides
2.6.1.43
8
-
L-alanine
pH 7.0, 37°C
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rhodobacter sphaeroides
-
5-aminolevulinate + pyruvate
-
Rhodobacter sphaeroides
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Rhodobacter sphaeroides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
partial
Rhodobacter sphaeroides
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
-15°C, stable for several months
Rhodobacter sphaeroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
delta-amino-n-valerate + 4,5-dioxopentanoate
transaminate at 95% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
2.6.1.43
epsilon-amino-n-caproate + 4,5-dioxopentanoate
transaminate at 103% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
2.6.1.43
gamma-amino-n-butyrate + 4,5-dioxopentanoate
transaminate at 122% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640045
Rhodobacter sphaeroides
5-aminolevulinate + pyruvate
-
640045
Rhodobacter sphaeroides
ir
2.6.1.43
L-ornithine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
2.6.1.43
additional information
ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity
640045
Rhodobacter sphaeroides
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
7
-
-
Rhodobacter sphaeroides
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.43
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM
Rhodobacter sphaeroides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
4,5-Dioxopentanoate
substrate inhibition
Rhodobacter sphaeroides
2.6.1.43
acetyl acetone
-
Rhodobacter sphaeroides
2.6.1.43
amino-oxyacetic acid
-
Rhodobacter sphaeroides
2.6.1.43
benzoyl acetone
-
Rhodobacter sphaeroides
2.6.1.43
beta-Alanine
8% inhibition
Rhodobacter sphaeroides
2.6.1.43
beta-Iodopropionate
30% inhibition
Rhodobacter sphaeroides
2.6.1.43
cadaverine
14% inhibition
Rhodobacter sphaeroides
2.6.1.43
cysteamine
-
Rhodobacter sphaeroides
2.6.1.43
cysteine
90% inhibition
Rhodobacter sphaeroides
2.6.1.43
diacetyl
-
Rhodobacter sphaeroides
2.6.1.43
ethyl acetoacetate
-
Rhodobacter sphaeroides
2.6.1.43
glyoxylic acid
-
Rhodobacter sphaeroides
2.6.1.43
homocysteine
-
Rhodobacter sphaeroides
2.6.1.43
iodoacetate
30% inhibition
Rhodobacter sphaeroides
2.6.1.43
Isonicotinic acid hydrazide
-
Rhodobacter sphaeroides
2.6.1.43
lysine
20% inhibition
Rhodobacter sphaeroides
2.6.1.43
additional information
iodoacetamide and chloroacetamide are not inhibitors
Rhodobacter sphaeroides
2.6.1.43
ophthalaldehyde
-
Rhodobacter sphaeroides
2.6.1.43
ornithine
20% inhibition
Rhodobacter sphaeroides
2.6.1.43
oxaloacetic acid
-
Rhodobacter sphaeroides
2.6.1.43
p-chloromercuribenzoate
78% inhibition
Rhodobacter sphaeroides
2.6.1.43
Penicillamine
-
Rhodobacter sphaeroides
2.6.1.43
Pyruvic acid
-
Rhodobacter sphaeroides
2.6.1.43
succinic acid
-
Rhodobacter sphaeroides
2.6.1.43
Thioglycollate
74% inhibition
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.4
-
4,5-Dioxopentanoate
pH 7.0, 37°C
Rhodobacter sphaeroides
2.6.1.43
8
-
L-alanine
pH 7.0, 37°C
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Rhodobacter sphaeroides
-
5-aminolevulinate + pyruvate
-
Rhodobacter sphaeroides
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
partial
Rhodobacter sphaeroides
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
-15°C, stable for several months
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
delta-amino-n-valerate + 4,5-dioxopentanoate
transaminate at 95% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
2.6.1.43
epsilon-amino-n-caproate + 4,5-dioxopentanoate
transaminate at 103% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
2.6.1.43
gamma-amino-n-butyrate + 4,5-dioxopentanoate
transaminate at 122% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640045
Rhodobacter sphaeroides
5-aminolevulinate + pyruvate
-
640045
Rhodobacter sphaeroides
ir
2.6.1.43
L-ornithine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
2.6.1.43
additional information
ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity
640045
Rhodobacter sphaeroides
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
7
-
-
Rhodobacter sphaeroides