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Literature summary extracted from

  • Yvon, M.; Chambellon, E.; Bolotin, A.; Roudot-Algaron, F.
    Characterization and role of the branched-chain aminotransferase (BcaT) isolated from Lactococcus lactis subsp. cremoris NCDO 763 (2000), Appl. Environ. Microbiol., 66, 571-577.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.6.1.42 nutrition widely used as starter in the cheese industry Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42
-
Lactococcus lactis
2.6.1.42 NCDO 763, gene cloned and sequenced with luciferase gene as the reporter Lactococcus lactis

General Stability

EC Number General Stability Organism
2.6.1.42 pure enzyme is not stable Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 Co2+
-
Lactococcus lactis
2.6.1.42 CoCl2
-
Lactococcus lactis
2.6.1.42 Cu2+
-
Lactococcus lactis
2.6.1.42 CuCl2
-
Lactococcus lactis
2.6.1.42 hydroxylamine
-
Lactococcus lactis
2.6.1.42 iodoacetamide
-
Lactococcus lactis
2.6.1.42 iodoacetic acid
-
Lactococcus lactis
2.6.1.42 phenylhydrazine
-
Lactococcus lactis
2.6.1.42 ZnCl2
-
Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 38000
-
2 * 38000, homodimer, SDS-PAGE Lactococcus lactis
2.6.1.42 64000
-
gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Lactococcus lactis
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Lactococcus lactis
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Lactococcus lactis
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Lactococcus lactis
-
subsp. cremoris NCDO 763 and TIL 46
-
2.6.1.42 Lactococcus lactis Q7BPX6 subsp. cremoris NCDO 763 and TIL 46, same nucleotide sequence accession number in DDBJ and EMBL >
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42
-
Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 94
-
substrate L-isoleucine + 2-oxoglutarate Lactococcus lactis

Storage Stability

EC Number Storage Stability Organism
2.6.1.42 -20°C, storage results in a very rapid loss of all activity Lactococcus lactis
2.6.1.42 0°C, storage results in a very rapid loss of all activity Lactococcus lactis
2.6.1.42 6°C, fairly stable for 1 week Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-alanine + pyruvate
-
Lactococcus lactis pyruvate + L-alanine
-
r
2.6.1.42 L-cysteine + 2-oxoglutarate
-
Lactococcus lactis 2-oxo-3-thiobutyrate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxo-isopentanoate
-
Lactococcus lactis 2-oxoisohexanoate + L-valine
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Lactococcus lactis 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoisohexanoate
-
Lactococcus lactis 3-methyl-2-oxopentanoate + L-leucine
-
r
2.6.1.42 L-isoleucine + 3-phenylpyruvate
-
Lactococcus lactis 2-oxoisohexanoate + L-phenylalanine
-
r
2.6.1.42 L-isoleucine + 4-methylthio-2-oxobutyrate
-
Lactococcus lactis 3-methyl-2-oxopentanoate + L-methionine
-
r
2.6.1.42 L-isoleucine + pyruvate
-
Lactococcus lactis 2-oxoisohexanoate + L-alanine
-
r
2.6.1.42 L-leucine + 2-oxo-isohexanoate
-
Lactococcus lactis 2-oxoisohexanoate + L-leucine
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Lactococcus lactis 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-methionine + 2-oxoglutarate
-
Lactococcus lactis 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
Lactococcus lactis phenylpyruvate + L-glutamate
-
r
2.6.1.42 L-tryptophan + 2-oxoglutarate
-
Lactococcus lactis 2-oxo-3-indolylpropanoate + L-glutamate
-
r
2.6.1.42 L-tyrosine + 2-oxoglutarate
-
Lactococcus lactis p-hydroxyphenylpyruvate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Lactococcus lactis 3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoisopentanoate
-
Lactococcus lactis 2-oxoisopentanoate + L-valine
-
r
2.6.1.42 additional information with 2-oxoglutarate as acceptor L-alanine is no substrate, with L-aspartate as amino donor, oxaloacetate is no amino acceptor Lactococcus lactis ?
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 dimer 2 * 38000, homodimer, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.42 35 40
-
Lactococcus lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.42 50 60 partially inactivated by heating at 50°, 40% inactivation after 30 min, more than 80% inactivation after 30 min at 60°C Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 7.5
-
-
Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
Lactococcus lactis