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Literature summary extracted from

  • Kagamiyama, H.; Hayashi, H.
    Branched-chain amino-acid aminotransferase of Escherichia coli (2000), Methods Enzymol., 324, 103-113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 cloning of ilv gene, Escherichia coli AB2227 transformed Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.42
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.24
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-tyrosine Escherichia coli
2.6.1.42 0.26
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-phenylalanine Escherichia coli
2.6.1.42 0.42
-
 L-isoleucine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
2.6.1.42 0.56
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-tryptophan Escherichia coli
2.6.1.42 0.89
-
 L-phenylalanine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
2.6.1.42 1
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-methionine Escherichia coli
2.6.1.42 1.7
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-valine Escherichia coli
2.6.1.42 2.2
-
 L-leucine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
2.6.1.42 2.4
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-isoleucine Escherichia coli
2.6.1.42 2.7
-
 L-isoleucine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
2.6.1.42 6.6
-
 2-oxoglutarate pH 8.0, 25°C, substrate L-leucine Escherichia coli
2.6.1.42 7
-
 L-tyrosine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
2.6.1.42 19
-
 L-methionine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
2.6.1.42 72
-
 L-tryptophan pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 33960
-
 6 * 33960 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Escherichia coli
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Escherichia coli last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Escherichia coli W3110 / ATCC 27325 last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Escherichia coli
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Escherichia coli
-
-
-
2.6.1.42 Escherichia coli W3110 / ATCC 27325
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 additional information
-
 final preparation, specific activity 23.9 U/mg Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Escherichia coli 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Escherichia coli W3110 / ATCC 27325 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine Escherichia coli W3110 / ATCC 27325 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-methionine + 2-oxoglutarate
-
 Escherichia coli 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
 Escherichia coli phenylpyruvate + L-glutamate
-
 r
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
 Escherichia coli W3110 / ATCC 27325 phenylpyruvate + L-glutamate
-
 r
2.6.1.42 L-tryptophan + 2-oxoglutarate
-
 Escherichia coli 2-oxo-3-indolylpropanoate + L-glutamate
-
 r
2.6.1.42 L-tyrosine + 2-oxoglutarate
-
 Escherichia coli p-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.42 L-tyrosine + 2-oxoglutarate
-
 Escherichia coli W3110 / ATCC 27325 p-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.42 L-valine + 2-oxoglutarate
-
 Escherichia coli 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 additional information no activity towards L-aspartate Escherichia coli ?
-
 ?
2.6.1.42 additional information no activity towards L-aspartate Escherichia coli W3110 / ATCC 27325 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 hexamer 6 * 33960 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.42 2.2
-
 L-tyrosine pH 8.0, 25°C Escherichia coli
2.6.1.42 2.9
-
 L-phenylalanine pH 8.0, 25°C Escherichia coli
2.6.1.42 3.7
-
 L-tryptophan pH 8.0, 25°C Escherichia coli
2.6.1.42 17
-
 L-methionine pH 8.0, 25°C Escherichia coli
2.6.1.42 19
-
 L-valine pH 8.0, 25°C Escherichia coli
2.6.1.42 48
-
 L-isoleucine pH 8.0, 25°C Escherichia coli
2.6.1.42 78
-
 L-leucine pH 8.0, 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Escherichia coli