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Literature summary extracted from

  • Aarnes, H.
    Branched chain amino acid aminotransferase in barley seedlings (1981), Z. Pflanzenphysiol., 102, 81-89.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.6.1.42 enzyme is more stable in phosphate buffer than in Tris-Cl buffer, addition of glycerol increases stability Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 2-oxo-3-methylvalerate measured in the forward reaction, concentrations higher than 1.0 mM inhibits the enzyme Hordeum vulgare
2.6.1.42 2-oxoisocaproate measured in the forward reaction, concentrations higher than 1.0 mM inhibits the enzyme Hordeum vulgare
2.6.1.42 2-oxoisovalerate measured in the forward reaction, concentrations higher than 1.0 mM inhibits the enzyme Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.1
-
2-Oxoisohexanoate pH 8.4, 30°C, glutamate as amino group donor Hordeum vulgare
2.6.1.42 0.2
-
2-oxo-3-methylpentanoate pH 8.4, 30°C Hordeum vulgare
2.6.1.42 0.4
-
2-oxoisopentanoate pH 8.4, 30°C Hordeum vulgare
2.6.1.42 0.8
-
L-leucine pH 8.4, 30°C Hordeum vulgare
2.6.1.42 0.8
-
2-oxoglutarate pH 8.4, 30°C, L-valine as amino group donor Hordeum vulgare
2.6.1.42 1.3
-
L-glutamate pH 8.4, 30°C, 2-oxoisocaproate as amino group acceptor Hordeum vulgare
2.6.1.42 1.5
-
2-oxoglutarate pH 8.4, 30°C, leucine as amino group donor Hordeum vulgare
2.6.1.42 1.8
-
2-oxoglutarate pH 8.4, 30°C, isoleucine as amino group donor Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 95000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Hordeum vulgare
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Hordeum vulgare
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Hordeum vulgare
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Hordeum vulgare
-
barley, L. cv. Ingrid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 partially Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.42 leaf
-
Hordeum vulgare
-
2.6.1.42 root
-
Hordeum vulgare
-
2.6.1.42 seedling
-
Hordeum vulgare
-
2.6.1.42 shoot
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 0.02
-
-
Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.6.1.42 -25°C, partially purified enzyme is stable for several weeks Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-alanine + 2-oxoglutarate
-
Hordeum vulgare 2-oxopropanoate + L-glutamate
-
r
2.6.1.42 L-alanine + 2-oxoisohexanoate
-
Hordeum vulgare pyruvate + L-leucine
-
r
2.6.1.42 L-alanine + 2-oxoisopentanoate
-
Hordeum vulgare pyruvate + L-valine
-
r
2.6.1.42 L-aspartate + 2-oxoglutarate
-
Hordeum vulgare oxaloacetate + L-glutamate
-
r
2.6.1.42 L-aspartate + 2-oxoisohexanoate
-
Hordeum vulgare oxaloacetate + L-leucine
-
r
2.6.1.42 L-aspartate + 2-oxoisopentanoate
-
Hordeum vulgare oxaloacetate + L-valine
-
r
2.6.1.42 L-cysteine + 2-oxoglutarate
-
Hordeum vulgare 2-oxo-3-thiobutyrate + L-glutamate
-
r
2.6.1.42 L-glutamate + 2-oxo-3-methylpentanoate
-
Hordeum vulgare 2-oxoglutarate + L-isoleucine
-
r
2.6.1.42 L-glutamate + 2-oxo-isohexanoate
-
Hordeum vulgare 2-oxoglutarate + L-leucine
-
r
2.6.1.42 L-glutamate + 2-oxo-isopentanoate
-
Hordeum vulgare 2-oxoglutarate + L-valine
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Hordeum vulgare 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + glyoxylate
-
Hordeum vulgare 2-oxoisohexanoate + glycine
-
r
2.6.1.42 L-isoleucine + oxaloacetate
-
Hordeum vulgare 2-oxoisohexanoate + L-aspartate
-
r
2.6.1.42 L-isoleucine + pyruvate
-
Hordeum vulgare 2-oxoisohexanoate + L-alanine
-
r
2.6.1.42 L-leucine + 2-oxo-3-methylpentanoate
-
Hordeum vulgare 2-oxoisohexanoate + L-isoleucine
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Hordeum vulgare 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoisohexanoate
-
Hordeum vulgare 2-oxoisohexanoate + L-leucine
-
r
2.6.1.42 L-leucine + 2-oxoisopentanoate
-
Hordeum vulgare 2-oxoisohexanoate + L-valine
-
r
2.6.1.42 L-leucine + glyoxylate
-
Hordeum vulgare 2-oxoisohexanoate + glycine
-
r
2.6.1.42 L-leucine + oxaloacetate
-
Hordeum vulgare 2-oxoisohexanoate + L-asparagine
-
r
2.6.1.42 L-leucine + pyruvate
-
Hordeum vulgare 2-oxoisohexanoate + L-alanine
-
r
2.6.1.42 L-methionine + 2-oxoglutarate transaminated extremely poorly Hordeum vulgare 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxo-3-methylpentanoate
-
Hordeum vulgare 3-methyl-2-oxobutanoate + L-isoleucine
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Hordeum vulgare 3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoisopentanoate
-
Hordeum vulgare 2-oxoisopentanoate + L-valine
-
r
2.6.1.42 L-valine + 4-methyl-2-oxopentanoate
-
Hordeum vulgare 2-oxoisopentanoate + L-leucine
-
r
2.6.1.42 L-valine + glyoxylate
-
Hordeum vulgare 2-oxoisopentanoate + glycine
-
r
2.6.1.42 L-valine + oxaloacetate
-
Hordeum vulgare 2-oxoisopentanoate + L-aspartate
-
r
2.6.1.42 L-valine + pyruvate
-
Hordeum vulgare 2-oxoisopentanoate + L-alanine
-
r
2.6.1.42 additional information no significant activity with glycine Hordeum vulgare ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 8.4
-
-
Hordeum vulgare
2.6.1.42 8.7
-
using valine in the reverse reaction Hordeum vulgare