EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | 2-mercaptoethanol | - |
Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | 2-oxoisocaproate | - |
Pseudomonas sp. | |
2.6.1.42 | Isocaproic acid | competitive inhibitor | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 0.3 | - |
2-oxoglutarate | pH 8.0, 30°C | Pseudomonas sp. | |
2.6.1.42 | 0.3 | - |
L-leucine | pH 8.0, 30°C | Pseudomonas sp. | |
2.6.1.42 | 1.1 | - |
2-Oxoisohexanoate | pH 8.0, 30°C | Pseudomonas sp. | |
2.6.1.42 | 1.2 | - |
L-leucine | pH 8.0, 30°C, activated with beta-mercaptoethanol | Pseudomonas sp. | |
2.6.1.42 | 3.2 | - |
L-glutamate | pH 8.0, 30°C | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-leucine + 2-oxoglutarate | Pseudomonas sp. | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | Pseudomonas sp. | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.42 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.42 | partially | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 2.5 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-2-aminobutyrate + 2-oxo-isopentanoate | - |
Pseudomonas sp. | 2-oxobutanoate + L-valine | - |
r | |
2.6.1.42 | L-2-aminobutyrate + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-2-aminobutyrate + pyruvate | - |
Pseudomonas sp. | 2-oxobutanoate + L-alanine | - |
r | |
2.6.1.42 | L-alanine + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxopropanoate + L-glutamate | - |
r | |
2.6.1.42 | L-arginine + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxo-5-guanidinopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-aspartate + 2-oxoglutarate | - |
Pseudomonas sp. | oxaloacetate + L-glutamate | - |
r | |
2.6.1.42 | L-glutamate + 2-oxo-isohexanoate | - |
Pseudomonas sp. | 2-oxoglutarate + L-leucine | - |
r | |
2.6.1.42 | L-glutamate + 2-oxo-isopentanoate | - |
Pseudomonas sp. | 2-oxoglutarate + L-valine | - |
r | |
2.6.1.42 | L-glutamate + 2-oxobutyrate | - |
Pseudomonas sp. | 2-oxoglutarate + 2-aminobutyrate | - |
r | |
2.6.1.42 | L-glutamate + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxoglutarate + L-glutamate | - |
r | |
2.6.1.42 | L-glutamate + pyruvate | - |
Pseudomonas sp. | 2-oxoglutarate + L-alanine | - |
r | |
2.6.1.42 | L-histidine + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxo-3-imidazolpropanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxo-isopentanoate | - |
Pseudomonas sp. | 2-oxoisohexanoate + L-valine | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxobutyrate | - |
Pseudomonas sp. | 2-oxoisohexanoate + 2-aminobutyrate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | lower reactivity than other origins | Pseudomonas sp. | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoisohexanoate | - |
Pseudomonas sp. | 3-methyl-2-oxopentanoate + L-leucine | - |
r | |
2.6.1.42 | L-isoleucine + pyruvate | - |
Pseudomonas sp. | 2-oxoisohexanoate + L-alanine | - |
r | |
2.6.1.42 | L-leucine + 2-oxo-butyrate | - |
Pseudomonas sp. | 2-oxoisohexanoate + 2-aminobutyrate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | - |
Pseudomonas sp. | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoisohexanoate | - |
Pseudomonas sp. | 2-oxoisohexanoate + L-leucine | - |
r | |
2.6.1.42 | L-leucine + pyruvate | - |
Pseudomonas sp. | 2-oxoisohexanoate + L-alanine | - |
r | |
2.6.1.42 | L-lysine + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxo-6-aminohexanoate + L-glutamate | - |
r | |
2.6.1.42 | L-methionine + 2-oxo-isohexanoate | - |
Pseudomonas sp. | 4-methylsulfanyl-2-oxobutanoate + L-leucine | - |
r | |
2.6.1.42 | L-methionine + 2-oxobutyrate | - |
Pseudomonas sp. | 4-methylsulfanyl-2-oxobutanoate + 2-aminobutyrate | - |
r | |
2.6.1.42 | L-methionine + 2-oxoglutarate | higher reactivity than other origins | Pseudomonas sp. | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-methionine + pyruvate | - |
Pseudomonas sp. | 4-methylsulfanyl-2-oxobutanoate + L-alanine | - |
r | |
2.6.1.42 | L-phenylalanine + 2-oxoglutarate | - |
Pseudomonas sp. | phenylpyruvate + L-glutamate | - |
r | |
2.6.1.42 | L-serine + 2-oxoglutarate | - |
Pseudomonas sp. | 3-hydroxy-2-oxopropanoate + L-glutamate | - |
r | |
2.6.1.42 | L-threonine + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxo-3-hydroxybutyrate + L-glutamate | - |
r | |
2.6.1.42 | L-tryptophan + 2-oxoglutarate | - |
Pseudomonas sp. | 2-oxo-3-indolylpropanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxo-isohexanoate | - |
Pseudomonas sp. | 2-oxoisopentanoate + L-leucine | - |
r | |
2.6.1.42 | L-valine + 2-oxobutyrate | - |
Pseudomonas sp. | 2-oxoisopentanoate + 2-aminobutanoate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | - |
Pseudomonas sp. | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + pyruvate | - |
Pseudomonas sp. | 2-oxoisopentanoate + L-alanine | - |
r | |
2.6.1.42 | additional information | L-glutamine is no substrate | Pseudomonas sp. | ? | - |
? |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 3.8 | - |
alpha-Ketoisocaproate | pH 8.0, 30°C | Pseudomonas sp. | |
2.6.1.42 | 6 | - |
Isocaproic acid | unactivated enzyme | Pseudomonas sp. | |
2.6.1.42 | 14 | - |
Isocaproic acid | activated enzyme | Pseudomonas sp. |