EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | 2-mercaptoethanol | 1.0 mM, relative activity 101% | Gluconobacter oxydans | |
2.6.1.42 | alpha,alpha'-dipyridyl | 1.0 mM, relative activity 103% | Gluconobacter oxydans | |
2.6.1.42 | EDTA | 1.0 mM, relative activity 108% | Gluconobacter oxydans | |
2.6.1.42 | glutathione | 1.0 mM, relative activity 109% | Gluconobacter oxydans | |
2.6.1.42 | neocuproin | 1.0 mM, relative activity 106% | Gluconobacter oxydans | |
2.6.1.42 | o-phenanthroline | 1.0 mM, relative activity 108% | Gluconobacter oxydans | |
2.6.1.42 | thioglycolate | 1.0 mM, relative activity 101% | Gluconobacter oxydans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | HgCl2 | - |
Gluconobacter oxydans | |
2.6.1.42 | hydroxylamine | - |
Gluconobacter oxydans | |
2.6.1.42 | p-chloromercuribenzoate | - |
Gluconobacter oxydans | |
2.6.1.42 | phenylhydrazine | - |
Gluconobacter oxydans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 0.33 | - |
2-oxoisopentanoate | pH 8.0, 37°C | Gluconobacter oxydans | |
2.6.1.42 | 0.91 | - |
2-Oxoisohexanoate | pH 8.0, 37°C | Gluconobacter oxydans | |
2.6.1.42 | 1.7 | - |
L-norleucine | pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor | Gluconobacter oxydans | |
2.6.1.42 | 1.82 | - |
L-leucine | pH 8.0, 37°C | Gluconobacter oxydans | |
2.6.1.42 | 2.5 | - |
2-oxo-3-methylpentanoate | pH 8.0, 37°C | Gluconobacter oxydans | |
2.6.1.42 | 3.03 | - |
L-isoleucine | pH 8.0, 37°C | Gluconobacter oxydans | |
2.6.1.42 | 4.57 | - |
2-oxoglutarate | pH 8.0, 37°C, leucine as amino group donor | Gluconobacter oxydans | |
2.6.1.42 | 4.7 | - |
L-norvaline | pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor | Gluconobacter oxydans | |
2.6.1.42 | 5.72 | - |
2-oxoglutarate | pH 8.0, 37°C, valine as amino group donor | Gluconobacter oxydans | |
2.6.1.42 | 6.45 | - |
L-valine | pH 8.0, 37°C, 2-oxoglutarate as amino group acceptor | Gluconobacter oxydans | |
2.6.1.42 | 6.67 | - |
2-oxoglutarate | pH 8.0, 37°C, isoleucine as amino group donor | Gluconobacter oxydans | |
2.6.1.42 | 10 | - |
L-aspartate | pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor | Gluconobacter oxydans | |
2.6.1.42 | 16.7 | - |
glutamate | pH 8.0, 37°C, leucine as amino group donor | Gluconobacter oxydans | |
2.6.1.42 | 18.2 | - |
L-glutamate | pH 8.0, 37°C, isoleucine as amino group donor | Gluconobacter oxydans | |
2.6.1.42 | 25 | - |
L-glutamate | pH 8.0, 37°C, valine as amino group donor | Gluconobacter oxydans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 80000 | - |
sedimentation equilibrium | Gluconobacter oxydans |
2.6.1.42 | 82000 | - |
gel filtration | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-isoleucine + 2-oxoglutarate | Gluconobacter oxydans | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | Gluconobacter oxydans | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | Gluconobacter oxydans | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.42 | Gluconobacter oxydans | - |
IFO 3172, synonym Gluconobacter suboxydans | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.42 | - |
Gluconobacter oxydans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 42.8 | - |
- |
Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-2-aminobutyrate + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-asparagine + 2-oxoglutarate | - |
Gluconobacter oxydans | 2,4-dioxo-4-aminobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-aspartate + 4-methyl-2-oxopentanoate | - |
Gluconobacter oxydans | ? | - |
? | |
2.6.1.42 | L-glutamine + 2-oxoglutarate | - |
Gluconobacter oxydans | 2,5-dioxo-5-aminopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Gluconobacter oxydans | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | - |
Gluconobacter oxydans | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + pyruvate | 2-oxoglutarate 100%, relative rate 1% | Gluconobacter oxydans | 2-oxoisohexanoate + L-alanine | - |
r | |
2.6.1.42 | L-methionine + 2-oxoglutarate | - |
Gluconobacter oxydans | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-norleucine + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxohexanoate + L-glutamate | - |
r | |
2.6.1.42 | L-norvaline + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-phenylalanine + 2-oxoglutarate | - |
Gluconobacter oxydans | phenylpyruvate + L-glutamate | - |
r | |
2.6.1.42 | L-tryptophan + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxo-3-indolylpropanoate + L-glutamate | - |
r | |
2.6.1.42 | L-tyrosine + 2-oxoglutarate | - |
Gluconobacter oxydans | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | - |
Gluconobacter oxydans | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | additional information | L-aspartic acid, L-arginine, L-citrulline, L-lysine, L-ornithine, L-alanine, beta-alanine and gamma-aminobutyrate are not amino donors, oxalaceteate and glyoxylate are not amino acceptors, pyruvate is a weak amino acceptor, relative activity 1% | Gluconobacter oxydans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.42 | alpha-KGA | - |
Gluconobacter oxydans |
2.6.1.42 | L-leucine-alpha-ketoglutarate transaminase | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 40 | 45 | - |
Gluconobacter oxydans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | - |
40 | stable at 0-30°C, rapidly inactivated over 40°C at pH 6.0 | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 9 | - |
- |
Gluconobacter oxydans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 5.5 | 6 | most stable in this range | Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | additional information | pyridoxine 5'-phosphate, pyridoxal, pyridoxamine and pyridoxine are not effective as coenzymes | Gluconobacter oxydans | |
2.6.1.42 | pyridoxal 5'-phosphate | Km 0.00053 mM | Gluconobacter oxydans | |
2.6.1.42 | pyridoxamine 5'-phosphate | Km 0.00062 mM | Gluconobacter oxydans |