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Literature summary extracted from

  • Aki, K.; Ogawa, K.; Ichihara, A.
    Transaminases of branched chain amino acids. IV. Purification and properties of two enzymes from rat liver (1968), Biochim. Biophys. Acta, 159, 276-284.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.42 2-mercaptoethanol
-
Rattus norvegicus
2.6.1.42 2-mercaptoethanol
-
Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.6 2-mercaptoethanol slight inhibition Rattus norvegicus
2.6.1.6 p-chloromercuribenzoate 1.0 mM, complete inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.6 0.065
-
2-oxoglutarate pH 8.2, 37°C Rattus norvegicus
2.6.1.6 25
-
leucine pH 8.2, 37°C Rattus norvegicus
2.6.1.42 0.75
-
L-leucine pH 8.2, 37°C Rattus norvegicus
2.6.1.42 0.84
-
L-isoleucine pH 8.2, 37°C Rattus norvegicus
2.6.1.42 1
-
2-oxoglutarate pH 8.2, 37°C Rattus norvegicus
2.6.1.42 4.3
-
L-valine pH 8.2, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.6 cytoplasm predominantly found in mitochondrion Rattus norvegicus 5737
-
2.6.1.6 microsome
-
Rattus norvegicus
-
-
2.6.1.6 mitochondrion
-
Rattus norvegicus 5739
-
2.6.1.6 nucleus
-
Rattus norvegicus 5634
-
2.6.1.42 cytoplasm
-
Rattus norvegicus 5737
-
2.6.1.42 cytoplasm
-
Sus scrofa 5737
-
2.6.1.42 microsome
-
Rattus norvegicus
-
-
2.6.1.42 mitochondrion
-
Sus scrofa 5739
-
2.6.1.42 mitochondrion mainly located in the cytoplasm Rattus norvegicus 5739
-
2.6.1.42 nucleus
-
Rattus norvegicus 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.6 L-leucine + 2-oxoglutarate Rattus norvegicus leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly 4-methyl-2-oxopentanoate + L-glutamate
-
?
2.6.1.42 L-isoleucine + 2-oxoglutarate Rattus norvegicus
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate Sus scrofa
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Sus scrofa
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Rattus norvegicus sole transaminase in fetal rat liver 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Rattus norvegicus
-
3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Sus scrofa
-
3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 additional information Rattus norvegicus specific for L-leucine, L-isoleucine or L-valine, no other amino acid would serve as an amino donor ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.6 Rattus norvegicus
-
-
-
2.6.1.42 Rattus norvegicus
-
rat, strain Wistar
-
2.6.1.42 Sus scrofa
-
pig, hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.6
-
Rattus norvegicus
2.6.1.42
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.6 liver
-
Rattus norvegicus
-
2.6.1.42 liver
-
Rattus norvegicus
-
2.6.1.42 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.6 1.033
-
-
Rattus norvegicus
2.6.1.42 0.01855
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.6 L-leucine + 2-oxoglutarate
-
Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.6 L-leucine + 2-oxoglutarate leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
?
2.6.1.6 L-leucine + oxaloacetate
-
Rattus norvegicus 4-methyl-2-oxopentanoate + L-aspartate
-
?
2.6.1.6 additional information specific for leucine, isoleucine or valine, no other amino acid would serve as an amino donor, pyruvate is no amino acid acceptor Rattus norvegicus ?
-
?
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Sus scrofa 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate sole transaminase in fetal rat liver Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Rattus norvegicus 3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Sus scrofa 3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 additional information specific for L-leucine, L-isoleucine or L-valine, no other amino acid would serve as an amino donor Rattus norvegicus ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.6 8.7
-
-
Rattus norvegicus
2.6.1.42 8.2
-
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.6 pyridoxal 5'-phosphate Km 0.004 mM Rattus norvegicus
2.6.1.42 pyridoxal 5'-phosphate Km 0.025 mM Rattus norvegicus
2.6.1.42 pyridoxal 5'-phosphate Km 0.0065 mM Sus scrofa