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Literature summary extracted from

  • Thompson, J.S.; Richardson, K.E.
    Isolation and characterization of a glutamate-glycine transaminase from human liver (1966), Arch. Biochem. Biophys., 117, 599-603.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.4 Cu2+ strong Homo sapiens
2.6.1.4 hydroxylamine no inhibition Homo sapiens
2.6.1.4 Isonicotinic acid hydrazide no inhibition Homo sapiens
2.6.1.4 Maleic acid no inhibition Homo sapiens
2.6.1.4 additional information no inhibition by CN- Homo sapiens
2.6.1.4 PCMB
-
Homo sapiens
2.6.1.4 phenylhydrazine pyridoxal phosphate restores activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.4 2
-
glyoxylate pH 7.7, 37°C Homo sapiens
2.6.1.4 2
-
glutamate pH 7.7, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.4 glyoxylate + L-glutamate Homo sapiens may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism glycine + 2-oxoglutarate
-
ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.4 290fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.4 liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.4 0.185
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.4 glyoxylate + L-alanine
-
Homo sapiens glycine + pyruvate
-
ir
2.6.1.4 glyoxylate + L-glutamate best substrate Homo sapiens glycine + 2-oxoglutarate
-
ir
2.6.1.4 glyoxylate + L-glutamate may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism Homo sapiens glycine + 2-oxoglutarate
-
ir
2.6.1.4 glyoxylate + L-glutamine
-
Homo sapiens glycine + 2-oxoglutaramate
-
ir
2.6.1.4 additional information substrate specificity Homo sapiens ?
-
?
2.6.1.4 additional information poor substrates are arginine or methionine Homo sapiens ?
-
?
2.6.1.4 additional information no activity with serine, valine, aspartate, histidine, phenylalanine, tyrosine, isoleucine Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.4 glutamate-glycine transaminase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.4 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.4 7.3
-
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.4 6.3 8.5 about half-maximal activity at pH 6.3 and about 60% of maximal activity at pH 8.5 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.4 pyridoxal 5'-phosphate required Homo sapiens
2.6.1.4 pyridoxal 5'-phosphate tightly bound to apoenzyme Homo sapiens
2.6.1.4 pyridoxal 5'-phosphate addition to enzyme system mostly without effect Homo sapiens