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Literature summary extracted from

  • Okuno, E.; Tsujimoto, M.; Nakamura, M.; Kido, R.
    2-Aminoadipate-2-oxoglutarate aminotransferase isoenzymes in human liver: a plausible physiological role in lysine and tryptophan metabolism (1993), Enzyme Protein, 47, 136-148.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.39 0.25
-
 L-2-aminoadipate pH 8.0, 37°C isoenzyme AadAT-II Homo sapiens
2.6.1.39 1
-
 2-oxoadipate pH 8.0, 37°C isoenzyme AadAT-II Homo sapiens
2.6.1.39 1.1
-
 2-oxoglutarate pH 8.0, 37°C isoenzyme AadAT-I Homo sapiens
2.6.1.39 1.4
-
 glutamate pH 8.0, 37°C Homo sapiens
2.6.1.39 2.5
-
 2-oxoadipate pH 8.0, 37°C isoenzyme AadAT-I Homo sapiens
2.6.1.39 3.2
-
 2-oxoglutarate pH 8.0, 37°C isoenzyme AadAT-II Homo sapiens
2.6.1.39 12.5
-
 L-glutamate pH 8.0, 37°C, isoenzyme AadAT-II Homo sapiens
2.6.1.39 20
-
 L-2-aminoadipate pH 8.0, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.39 cytoplasm isoenzyme AadAT-I Homo sapiens 5737
-
2.6.1.39 cytosol
-
 Homo sapiens 5829
-
2.6.1.39 mitochondrion isoenzyme AadAT-II Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.39 46000
-
 2 * 46000, SDS-PAGE Homo sapiens
2.6.1.39 98000
-
 isoenzyme AadAT-II, sucrose density gradient centrifugation Homo sapiens
2.6.1.39 104000
-
 isoenzyme AadAT-I, sucrose density gradient centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate Homo sapiens lysine and tryptophan metabolism 2-oxoadipate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.39 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.39 2 isoenzymes, AadAT-I and AadAT-II Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.39 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.39 0.77
-
 isoenzyme AadAT-I Homo sapiens
2.6.1.39 10.68
-
 isoenzyme AadAT-II Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.39 kynurenine + 2-oxoglutarate
-
 Homo sapiens kynurenic acid + L-glutamate
-
 ir
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate
-
 Homo sapiens 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate lysine and tryptophan metabolism Homo sapiens 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-tryptophan + 2-oxoglutarate
-
 Homo sapiens 3-indole-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.39 additional information 2 isoenzymes, AadAT-I and AadAT-II, isoenzyme AadAT-II shows additional activity with tryptophan or kynurenine-2-oxoglutarate reaction, only slight activity with asparagine, alanine, arginine, ornithine, isoleucine, valine, leucine, lysine, serine, threonine, phenylalanine, tyrosine, histidine, glutamine, methionine or aspartate with 2-oxoglutarate Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.39 dimer 2 * 46000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.6.1.39 AadAT
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.39 9 9.5 isoenzyme AadAT-II Homo sapiens
2.6.1.39 9.5
-
 isoenzyme AadAT-I Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.39 9 9.5 isoenzyme AadAT-I, sensitive to pH changes, activity is quickly lost at a higher pH, isoenzyme AadAT-II is less sensitive to pH changes Homo sapiens