EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.30 | additional information | phosphate is neither essential nor inhibitory | Pseudomonas sp. |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.6.1.30 | addition of saturated ammonium sulfate solution to the concentrated protein solution until the first permanent turbidity appears, several days at 5°C | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.30 | additional information | phosphate is neither essential nor inhibitory | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.30 | 0.043 | - |
pyridoxamine | pH 8.5, 37°C | Pseudomonas sp. | |
2.6.1.30 | 0.1 | - |
pyruvate | pH 8.5, 37°C | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.30 | pyridoxamine + pyruvate | Pseudomonas sp. | oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor | pyridoxal + L-alanine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.30 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.30 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.30 | 7.3 | - |
purified enzyme | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.30 | pyridoxamine + pyruvate | oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor | Pseudomonas sp. | pyridoxal + L-alanine | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.30 | 37 | - |
assay at | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.30 | 70 | - |
10 min, stable | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.30 | additional information | - |
additional information | - |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.30 | 8.5 | - |
- |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.30 | additional information | pyridoxal 5'-phosphate is not required for activity | Pseudomonas sp. |