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Literature summary extracted from

  • Thibert, R.J.; Schmidt, D.E.
    Purification and partial characterization of cysteine-glutamate transaminase from rat liver (1977), Can. J. Biochem., 55, 958-964.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.3 alpha-Methyl-DL-cysteine inhibits transamination of cysteine Rattus norvegicus
2.6.1.3 Maleate
-
 Rattus norvegicus
2.6.1.3 sodium phosphate buffer
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.3 83500
-
 gel filtration Rattus norvegicus
2.6.1.3 84000
-
 1 * 84000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.3 L-cysteine + 2-oxoglutarate Rattus norvegicus important part of enzyme together with beta-mercaptopyruvate sulfurtransferase in the regulation of thiosulfate formation L-glutamate + beta-mercaptopyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.3 TEAE-cellulose, isoelectric focusing Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.3 2.085
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.3 -20ºC, 68% inactivated after 32 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.3 L-cysteine + 2-oxoglutarate
-
 Rattus norvegicus L-glutamate + beta-mercaptopyruvate
-
 ?
2.6.1.3 L-cysteine + 2-oxoglutarate important part of enzyme together with beta-mercaptopyruvate sulfurtransferase in the regulation of thiosulfate formation Rattus norvegicus L-glutamate + beta-mercaptopyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.3 monomer 1 * 84000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.3 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.3 additional information
-
 1 h at room temperature, complete inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.3 8.2
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.3 7 11 about 60% of activity maximum at pH 7 and 11 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.3 pyridoxal 5'-phosphate pyridoxal phosphate protein Rattus norvegicus