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Literature summary extracted from

  • O'Neil, S.R.; DeMoss, R.D.
    Tryptophan transaminase from Clostridium sporogenes (1968), Arch. Biochem. Biophys., 127, 361-369.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.27 additional information high salt concentrations activate Clostridium sporogenes
2.6.1.27 additional information no activation by: pyridoxine, pyridoxal, pyridoxamine Clostridium sporogenes

General Stability

EC Number General Stability Organism
2.6.1.27 low buffer concentrations: loss of activity Clostridium sporogenes
2.6.1.27 unstable upon freezing and thawing Clostridium sporogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.27 0.158
-
 2-oxoglutarate cosubstrate phenylalanine , pH 8.4, 22°C approximately Clostridium sporogenes
2.6.1.27 2.68
-
 L-tryptophan cosubstrate tryptophan, pH 8.4, 22°C approximately Clostridium sporogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.27 97000
-
 sucrose density gradient centrifugation Clostridium sporogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.27 L-tryptophan + 2-oxoglutarate Clostridium sporogenes first step in metabolic path for the conversion of L-tryptophan to indolepropionate L-glutamate + 3-indole-2-oxopropanoate
-
 ?
2.6.1.27 L-tryptophan + 2-oxoglutarate Clostridium sporogenes 175 first step in metabolic path for the conversion of L-tryptophan to indolepropionate L-glutamate + 3-indole-2-oxopropanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.27 Clostridium sporogenes
-
-
-
2.6.1.27 Clostridium sporogenes 175
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.27 partial, 200fold Clostridium sporogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.27 23.6
-
 partially purified enzyme Clostridium sporogenes

Storage Stability

EC Number Storage Stability Organism
2.6.1.27 0°C, partially purified enzyme, stable for at least 48 h Clostridium sporogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.27 L-phenylalanine + 2-oxoglutarate
-
 Clostridium sporogenes L-glutamate + phenylpyruvate
-
 ?
2.6.1.27 L-phenylalanine + 2-oxoglutarate
-
 Clostridium sporogenes 175 L-glutamate + phenylpyruvate
-
 ?
2.6.1.27 L-tryptophan + 2-oxoglutarate
-
 Clostridium sporogenes L-glutamate + 3-indole-2-oxopropanoate
-
 ?
2.6.1.27 L-tryptophan + 2-oxoglutarate first step in metabolic path for the conversion of L-tryptophan to indolepropionate Clostridium sporogenes L-glutamate + 3-indole-2-oxopropanoate
-
 ?
2.6.1.27 L-tryptophan + 2-oxoglutarate
-
 Clostridium sporogenes 175 L-glutamate + 3-indole-2-oxopropanoate
-
 ?
2.6.1.27 L-tryptophan + 2-oxoglutarate first step in metabolic path for the conversion of L-tryptophan to indolepropionate Clostridium sporogenes 175 L-glutamate + 3-indole-2-oxopropanoate
-
 ?
2.6.1.27 L-tyrosine + 2-oxoglutarate
-
 Clostridium sporogenes L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
 ?
2.6.1.27 L-tyrosine + 2-oxoglutarate
-
 Clostridium sporogenes 175 L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.27 22
-
 assay at Clostridium sporogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.27 8.4
-
-
 Clostridium sporogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.27 7 9.3 pH 7.0: about 55% of activity maximum, pH 9.3: about 60% of activity maximum Clostridium sporogenes

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.27 pyridoxal 5'-phosphate Km: 0.00218 mM Clostridium sporogenes
2.6.1.27 pyridoxal 5'-phosphate required for full activation Clostridium sporogenes
2.6.1.27 pyridoxamine 5'-phosphate activates Clostridium sporogenes