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Literature summary extracted from

  • Huynh, Q.K.; Gulve, E.A.; Dian, T.
    Purification and characterization of glutamine:fructose 6-phosphate amidotransferase from rat liver (2000), Arch. Biochem. Biophys., 379, 307-313.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.16 extremely unstable during purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.16 4,4'-dithiodipyridine inactivation reversed by dithiothreitol. Competitive with respect to L-glutamine. Non-competitive with respect to D-fructose 6-phosphate Rattus norvegicus
2.6.1.16 5,5'-dithionitrobenzoic acid inactivation reversed by dithiothreitol Rattus norvegicus
2.6.1.16 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
2.6.1.16 UDP-N-acetylglucosamine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.16 0.4
-
 D-fructose 6-phosphate
-
 Rattus norvegicus
2.6.1.16 0.8
-
 L-glutamine
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.16 75000
-
 4 * 75000, gel filtration, SDS-PAGE Rattus norvegicus
2.6.1.16 280000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.16 0.866
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.16 -80°C, 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Rattus norvegicus L-glutamate + D-glucosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.16 tetramer 4 * 75000, gel filtration, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.16 7.5
-
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.16 0.0025
-
 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
2.6.1.16 0.0183
-
 UDP-N-acetylglucosamine
-
 Rattus norvegicus