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Literature summary extracted from

  • Tanaka, T.; Tokuda, T.; Tachibana, A.; Taniguchi, M.; Oi, S.
    Purification and some properties of aspartate aminotransferase of Methanobacterium thermoformicicum SF-4 (1990), Agric. Biol. Chem., 54, 625-631.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 2-oxoglutarate competitive to L-aspartate Methanothermobacter thermautotrophicus
2.6.1.1 adipate moderately Methanothermobacter thermautotrophicus
2.6.1.1 fumarate weak Methanothermobacter thermautotrophicus
2.6.1.1 Glutarate weak Methanothermobacter thermautotrophicus
2.6.1.1 Maleate
-
 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 50000
-
 1 * 50000, SDS-PAGE Methanothermobacter thermautotrophicus
2.6.1.1 57000
-
 gel filtration, gradient PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus SF-4
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Methanothermobacter thermautotrophicus
-
-
-
2.6.1.1 Methanothermobacter thermautotrophicus SF-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 8.44
-
 purified enzyme Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.1 -20°C or 4°C, stable for at least 2 weeks Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate 25.2% of the activity with glutamate Methanothermobacter thermautotrophicus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate 25.2% of the activity with glutamate Methanothermobacter thermautotrophicus SF-4 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-cysteine sulfinic acid + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-cysteine sulfinic acid + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Methanothermobacter thermautotrophicus 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Methanothermobacter thermautotrophicus SF-4 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-methionine + 2-oxoglutarate 1.0% of the activity with glutamate Methanothermobacter thermautotrophicus L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
2.6.1.1 additional information substrate specificity Methanothermobacter thermautotrophicus ?
-
 ?
2.6.1.1 additional information substrate specificity Methanothermobacter thermautotrophicus SF-4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 monomer 1 * 50000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 60
-
-
 Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 70
-
 pH 7.5, 1 h, stable up to Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 8.5
-
-
 Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.1 7 11
-
 Methanothermobacter thermautotrophicus