EC Number | General Stability | Organism |
---|---|---|
2.6.1.1 | 2-oxoglutarate does not protect against heat inactivation | Chlamydomonas reinhardtii |
2.6.1.1 | addition of glycerol, DTT and pyridoxal 5'-phosphate to buffers, required for stabilization during purification | Chlamydomonas reinhardtii |
2.6.1.1 | aspartate, 10 mM, or pyridoxal 5'-phosphate, 0.2 mM protects against heat inactivation | Chlamydomonas reinhardtii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.1 | CN- | - |
Chlamydomonas reinhardtii | |
2.6.1.1 | L-ascorbate | - |
Chlamydomonas reinhardtii | |
2.6.1.1 | Methylacetimidate | complete inhibition at 50 mM | Chlamydomonas reinhardtii | |
2.6.1.1 | additional information | no substrate inhibition | Chlamydomonas reinhardtii | |
2.6.1.1 | p-hydroxymercuribenzoate | - |
Chlamydomonas reinhardtii | |
2.6.1.1 | Thiosemicarbazide | - |
Chlamydomonas reinhardtii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.1 | 0.044 | - |
oxaloacetate | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
2.6.1.1 | 0.55 | - |
2-oxoglutarate | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
2.6.1.1 | 2.53 | - |
L-aspartate | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
2.6.1.1 | 3.88 | - |
L-glutamate | pH 7.8, 25°C | Chlamydomonas reinhardtii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 65000 | - |
2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
2.6.1.1 | 138000 | - |
gel filtration | Chlamydomonas reinhardtii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | Chlamydomonas reinhardtii | - |
oxaloacetate + L-glutamate | - |
? | |
2.6.1.1 | L-aspartate + 2-oxoglutarate | Chlamydomonas reinhardtii 6145c | - |
oxaloacetate + L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.1 | Chlamydomonas reinhardtii | - |
- |
- |
2.6.1.1 | Chlamydomonas reinhardtii 6145c | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.1 | 400fold | Chlamydomonas reinhardtii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Chlamydomonas reinhardtii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 84 | - |
purified enzyme | Chlamydomonas reinhardtii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | - |
Chlamydomonas reinhardtii | oxaloacetate + L-glutamate | - |
? | |
2.6.1.1 | L-aspartate + 2-oxoglutarate | - |
Chlamydomonas reinhardtii 6145c | oxaloacetate + L-glutamate | - |
? | |
2.6.1.1 | L-phenylalanine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-phenylalanine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii 6145c | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tyrosine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tyrosine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii 6145c | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.1 | additional information | substrate specificity | Chlamydomonas reinhardtii | ? | - |
? | |
2.6.1.1 | additional information | substrate specificity | Chlamydomonas reinhardtii 6145c | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.1 | dimer | 2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 37 | - |
- |
Chlamydomonas reinhardtii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 60 | - |
5 min, more than 90% loss of activity | Chlamydomonas reinhardtii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 7.8 | - |
- |
Chlamydomonas reinhardtii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.1 | pyridoxal 5'-phosphate | 2 mol of pyridoxal phosphate per mol of enzyme | Chlamydomonas reinhardtii | |
2.6.1.1 | pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Chlamydomonas reinhardtii |