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Literature summary extracted from

  • Tanaka, T.; Yamamoto, S.; Taniguchi, M.; Hayashi, H.; Kuramitsu, S.; Kagamiyama, H.; Oi, S.
    Further studies on aspartate aminotransferase of thermophilic methanogens by analysis of general properties, bound cofactors, and subunit structures (1992), J. Biochem., 112, 811-815.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 43000
-
 4 * 43000, SDS-PAGE Methanothermobacter thermautotrophicus
2.6.1.1 180000
-
 gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Methanothermobacter thermautotrophicus SF-4
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Methanothermobacter thermautotrophicus
-
-
-
2.6.1.1 Methanothermobacter thermautotrophicus
-
 strains FTF-INRA, DSM 3012
-
2.6.1.1 Methanothermobacter thermautotrophicus
-
 DSM 6457
-
2.6.1.1 Methanothermobacter thermautotrophicus SF-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 21.8
-
 purified enzyme Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate
-
 Methanothermobacter thermautotrophicus SF-4 2-oxoglutarate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 tetramer 4 * 43000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 60
-
-
 Methanothermobacter thermautotrophicus
2.6.1.1 70
-
 strain FTF-INRA Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 40
-
 pH 7.5, 1 h, gradual loss of activity above Methanothermobacter thermautotrophicus
2.6.1.1 70
-
 pH 7.5, 1 h, stable up to Methanothermobacter thermautotrophicus
2.6.1.1 70
-
 pH 7.5, 1 h, enzyme loses 65% of activity Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 8.5
-
-
 Methanothermobacter thermautotrophicus