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Literature summary extracted from

  • Marino, G.; Nitti, G.; Arnone, M.I.; Sannia, G.; Gambacorta, A.; De Rosa, M.
    Purification and characterization of aspartate aminotransferase from the thermoacidophilic archaebacterium Sulfolobus solfataricus (1988), J. Biol. Chem., 263, 12305-12309.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 53000
-
 2 * 53000, SDS-PAGE Saccharolobus solfataricus
2.6.1.1 53000
-
 2 * 53000 Saccharolobus solfataricus
2.6.1.1 108000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Saccharolobus solfataricus
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Saccharolobus solfataricus
-
-
-
2.6.1.1 Saccharolobus solfataricus P14909
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1
-
 Saccharolobus solfataricus
2.6.1.1 840fold Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.1 L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Saccharolobus solfataricus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.6.1.1 reconstitution of holoenzyme with pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 84
-
 purified enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Saccharolobus solfataricus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate 48% of the activity with cysteine sulfinic acid Saccharolobus solfataricus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-cysteine sulfinic acid + 2-oxoglutarate
-
 Saccharolobus solfataricus 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate 25% of the activity with cysteine sulfinic acid Saccharolobus solfataricus 2-oxoglutarate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 dimer 2 * 53000, SDS-PAGE Saccharolobus solfataricus
2.6.1.1 dimer 2 * 53000 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 60
-
 assay at Saccharolobus solfataricus
2.6.1.1 95
-
 above Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.1 25 95
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 90
-
 6 h, stable Saccharolobus solfataricus
2.6.1.1 100
-
 t1/2: 2 h Saccharolobus solfataricus
2.6.1.1 100
-
 2 h, 50% inactivation Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 8.5
-
 assay at Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.1 5.5 9.5 no definite pH optimum Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.1 5
-
 irreversible inactivation Saccharolobus solfataricus
2.6.1.1 6 9 stable Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Saccharolobus solfataricus
2.6.1.1 pyridoxal 5'-phosphate 1 mol of pyridoxal phosphate per mol of subunit Saccharolobus solfataricus
2.6.1.1 pyridoxal 5'-phosphate apoenzyme can be reconstituted with pyridoxamine 5”phosphate and/or pyridoxal 5'-phosphate, each subunit binding 1 mol of coenzym Saccharolobus solfataricus
2.6.1.1 pyridoxamine 5'-phosphate 1 mol per mol of subunit Saccharolobus solfataricus
2.6.1.1 pyridoxamine 5'-phosphate reverse reaction Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Saccharolobus solfataricus microheterogenity of purified enzyme 6.8 6.3
2.6.1.1 Saccharolobus solfataricus microheterogeneity when submitted to chromatofocusing and/or isoelectric focusing analysis. Two main bands having pI = 6.8 and 6.3 are observed
-
 6.3
2.6.1.1 Saccharolobus solfataricus microheterogeneity when submitted to chromatofocusing and/or isoelectric focusing analysis. Two main bands having pI = 6.8 and 6.3 are observed
-
 6.8