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Literature summary extracted from

  • Orlacchio, A.; Campos-Cavieres, M.; Pashev, I.; Mun, E.A.
    Some kinetic and other properties of the isoenzymes of aspartate aminotransferase isolated from sheep liver (1979), Biochem. J., 177, 583-593.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 2-oxoglutarate cytoplasmic enzyme inhibited above 0.25 mM, mitochondrial enzyme not Ovis aries
2.6.1.1 phosphate inhibition of cofactor binding to the apoenzyme, cytosolic and mitochondrial isozymes Ovis aries
2.6.1.1 potassium phosphate
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 additional information
-
 additional information kinetics Ovis aries
2.6.1.1 0.093
-
 2-oxoglutarate cytosolic isozyme, pH 7.4, 25°C Ovis aries
2.6.1.1 0.098
-
 2-oxoglutarate mitochondrial isozyme, pH 7.4, 25°C Ovis aries
2.6.1.1 0.4
-
 L-aspartate mitochondrial isozyme, pH 7.4, 25°C Ovis aries
2.6.1.1 3
-
 L-aspartate cytosolic isozyme, pH 7.4, 25°C Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.1 cytosol
-
 Ovis aries 5829
-
2.6.1.1 mitochondrion
-
 Ovis aries 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 87100
-
 sedimentation equilibrium analysis Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Ovis aries
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Ovis aries
-
 cytosolic and mitochondrial isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 mitochondrial isozyme, 57.3fold Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 151.5
-
 mitochondrial isozyme Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Ovis aries oxaloacetate + L-glutamate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 6.5 8.5
-
 Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Ovis aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.1 0.0195
-
 phosphate mitochondrial isozyme, pH 7.4, 37°C Ovis aries
2.6.1.1 0.032
-
 phosphate cytosolic isozyme, pH 7.4, 37°C Ovis aries