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Literature summary extracted from

  • Recasens, M.; Benezra, R.; Mandel, P.
    Cysteine sulfinate aminotransferase and aspartate aminotransferase isoenzymes of rat brain. Purification, characterization, and further evidence for identity (1980), Biochemistry, 19, 4583-4589.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.1 cysteinic acid protects the cytosolic isoform against thermal denaturation, not aspartate or 2-oxoglutarate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 2-oxoglutarate competitive to cysteine sulfinic acid or aspartate Rattus norvegicus
2.6.1.1 gamma-Acetylenic GABA
-
 Rattus norvegicus
2.6.1.1 L-aspartate weak, competitive against 2-oxoglutarate Rattus norvegicus
2.6.1.1 L-cysteine sulfinic acid weak, competitive to 2-oxoglutarate Rattus norvegicus
2.6.1.1 L-cysteinic acid
-
 Rattus norvegicus
2.6.1.1 L-serine O-sulfate inhibition of cytosolic enzyme, no inhibition of mitochondrial enzyme Rattus norvegicus
2.6.1.1 additional information no inhibition by dipropylacetic acid, vinyl-GABA Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.1 cytosol
-
 Rattus norvegicus 5829
-
2.6.1.1 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 41000
-
 2 * 41000, cytosolic isozyme, SDS-PAGE Rattus norvegicus
2.6.1.1 43400
-
 2 * 43400, mitochondrial isozyme, SDS-PAGE Rattus norvegicus
2.6.1.1 84000
-
 cytosolic isozyme, gel filtration Rattus norvegicus
2.6.1.1 86000
-
 mitochondrial isozyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Rattus norvegicus
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Rattus norvegicus
-
 cytosolic and mitochondrial isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 mitochondrial and cytosolic isozymes Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.1 L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 202
-
 purified mitochondrial isozyme Rattus norvegicus
2.6.1.1 215
-
 purified cytosolic isozyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 2,4-diaminobutyric acid + 2-oxoglutarate
-
 Rattus norvegicus ?
-
 ?
2.6.1.1 2-aminohexanedioic acid + 2-oxoglutarate i.e. alpha-aminoadipic acid Rattus norvegicus 2-oxohexanedioic acid + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Rattus norvegicus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate best amino acid donor of cytosolic enzyme Rattus norvegicus oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-cysteic acid + 2-oxoglutarate i.e. 2-amino-2-sulfopropionate Rattus norvegicus 2-oxo-3-sulfopropionate + L-glutamate
-
 ?
2.6.1.1 L-cysteine sulfinic acid + 2-oxoglutarate best amino acid donor for mitochondrial enzyme Rattus norvegicus 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-tryptophan + 2-oxoglutarate
-
 Rattus norvegicus 3-indole-2-oxopropionic acid + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 dimer 2 * 43400, mitochondrial isozyme, SDS-PAGE Rattus norvegicus
2.6.1.1 dimer 2 * 41000, cytosolic isozyme, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 additional information
-
 cysteine sulfinic acid protects against heat inactivation Rattus norvegicus
2.6.1.1 50
-
 15 min, 10% loss of activity for the mitochondrial isozyme, 80% loss of activity with aspartate for the cytosolic isozyme Rattus norvegicus
2.6.1.1 70
-
 15 min, mitochondrial enzyme completely destroyed Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 8.5 10 cytosolic enzyme Rattus norvegicus
2.6.1.1 8.7
-
 mitochondrial enzyme Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.1 7.4 10 pH 7.4: 60% of activity maximum, pH 8.5-10.0: activity maximum, cytosolic enzyme Rattus norvegicus
2.6.1.1 7.4 8.7 pH 7.4: 95% of activity maximum, pH 8.7: activity maximum, mitochondrial enzyme Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.1 2
-
 irreversibly destroyed below Rattus norvegicus
2.6.1.1 3.3
-
 1 h, 10% loss of activity of cytoplasmic enzyme, 40% loss of activity of mitochondrial enzyme Rattus norvegicus
2.6.1.1 12
-
 irreversibly destroyed above Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.1 0.96
-
 2-oxoglutarate cytosolic isozyme, pH 8.6, versus cysteinic acid Rattus norvegicus
2.6.1.1 1.9
-
 2-oxoglutarate mitochondrial isozyme, pH 8.6, versus L-aspartate Rattus norvegicus
2.6.1.1 2.5
-
 2-oxoglutarate mitochondrial isozyme, pH 8.6, versus cysteinic acid Rattus norvegicus
2.6.1.1 5.1
-
 L-cysteinic acid cytosolic isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus
2.6.1.1 17
-
 2-oxoglutarate cytosolic isozyme, pH 8.6, versus L-aspartate Rattus norvegicus
2.6.1.1 21
-
 aspartate cytosolic isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus
2.6.1.1 26
-
 L-cysteinic acid mitochondrial isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus
2.6.1.1 263
-
 aspartate mitochondrial isozyme, pH 8.6, versus 2-oxoglutarate Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Rattus norvegicus cytoslic isozyme
-
 5.7
2.6.1.1 Rattus norvegicus mitochondrial isozyme
-
 9.1