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Literature summary extracted from

  • Lowe, P.N.; Rowe, A.F.
    Aspartate: 2-oxoglutarate aminotransferase from trichomonas vaginalis. Identity of aspartate aminotransferase and aromatic amino acid aminotransferase (1985), Biochem. J., 232, 689-695.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 2-oxoglutarate substrate inhibition at high concentration Trichomonas vaginalis
2.6.1.1 adipate no inhibition Trichomonas vaginalis
2.6.1.1 L-glutamate competitive Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.062
-
2-oxoglutarate 30°C Trichomonas vaginalis
2.6.1.1 0.96
-
L-aspartate 30°C Trichomonas vaginalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 44000
-
2 * 44000, mitochondria, SDS-PAGE Trichomonas vaginalis
2.6.1.1 100000
-
gel filtration Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Trichomonas vaginalis
-
oxaloacetate + L-glutamate
-
?
2.6.1.1 L-aspartate + 2-oxoglutarate Trichomonas vaginalis Bushby
-
oxaloacetate + L-glutamate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Trichomonas vaginalis
-
Bushby strain
-
2.6.1.1 Trichomonas vaginalis Bushby
-
Bushby strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 200-1000fold, copurification with the aromatic amino acid aminotransferase 2.6.1.57 Trichomonas vaginalis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.1 L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Trichomonas vaginalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 172
-
purified enzyme Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-alanine + 2-oxoglutarate
-
Trichomonas vaginalis pyruvate + L-glutamate
-
?
2.6.1.1 L-alanine + 2-oxoglutarate
-
Trichomonas vaginalis Bushby pyruvate + L-glutamate
-
?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
Trichomonas vaginalis oxaloacetate + L-glutamate
-
?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
Trichomonas vaginalis oxaloacetate + L-glutamate
-
r
2.6.1.1 L-aspartate + 2-oxoglutarate
-
Trichomonas vaginalis Bushby oxaloacetate + L-glutamate
-
?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
Trichomonas vaginalis Bushby oxaloacetate + L-glutamate
-
r
2.6.1.1 L-phenylalanine + 2-oxoglutarate
-
Trichomonas vaginalis 2-oxo-3-phenylpropionic acid + L-glutamate i.e. phenylpyruvate ?
2.6.1.1 L-tryptophan + 2-oxoglutarate
-
Trichomonas vaginalis 3-indole-2-oxopropionic acid + L-glutamate
-
?
2.6.1.1 L-tyrosine + 2-oxoglutarate
-
Trichomonas vaginalis 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate i.e. 4-hydroxyphenylpyruvate ?
2.6.1.1 additional information substrate specificity Trichomonas vaginalis ?
-
?
2.6.1.1 additional information no activity with L-cysteine, L-isoleucine, L-leucine, L-valine, L-glutamine, glycine, L-ornithine, L-lysine, L-arginine Trichomonas vaginalis ?
-
?
2.6.1.1 additional information substrate specificity Trichomonas vaginalis Bushby ?
-
?
2.6.1.1 additional information no activity with L-cysteine, L-isoleucine, L-leucine, L-valine, L-glutamine, glycine, L-ornithine, L-lysine, L-arginine Trichomonas vaginalis Bushby ?
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 dimer 2 * 44000, mitochondria, SDS-PAGE Trichomonas vaginalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.1 2.8
-
L-glutamate 30°C, competitive versus L-aspartate Trichomonas vaginalis
2.6.1.1 6.8
-
L-glutamate 30°C, competitive versus 2-oxoglutrate Trichomonas vaginalis
2.6.1.1 30
-
2-oxoglutarate 30°C, substrate inhibition Trichomonas vaginalis