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Literature summary extracted from

  • Schmid, H.C.; Oster, U.; Kogel, J.; Lenz, S.; Rüdiger, W.
    Cloning and characterisation of chlorophyll synthase from Avena sativa (2001), Biol. Chem., 382, 903-911.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.62 expression in Escherichia coli Avena sativa

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.62 C109A mutant enzyme exhibits nearly no enzymatic activity, N-phenylmaleimide results in an additional decrease of activity Avena sativa
2.5.1.62 C130A mutant enzyme shows reduced activity and sensitivity to N-phenylmaleimide Avena sativa
2.5.1.62 C137A mutant enzyme is not impaired in enzymatic activity and shows the same inhibition by N-phenylmaleimide as the wild-type enzyme Avena sativa
2.5.1.62 C262A mutant enzyme is not impaired in enzymatic activity and shows the same inhibition by N-phenylmaleimide as the wild-type enzyme Avena sativa
2.5.1.62 C304A as active as the wild-type enzyme, mutant enzyme is not inhibited by N-phenylmaleimide Avena sativa
2.5.1.62 additional information deletion of the presequence yields a protein with full activity, even further deletion of the N-terminus including amino acid residues 1-87 results in a core protein that is still enzymatically active. Deletion of the 88 N-terminal residues yields a protein without enzymatic activity. At the C-terminus, only one residue H378 can be deleted without loss of activity, while deletion of S77 together with H378, and all shorter sequences show no activity Avena sativa
2.5.1.62 R151A mutant enzyme with 35% of the activity compared to wild-type enzyme Avena sativa
2.5.1.62 R161A mutant enzyme without activity Avena sativa
2.5.1.62 R284A mutant enzyme shows wild-type activity Avena sativa
2.5.1.62 R91A mutant enzyme without activity Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.62 diacetyl
-
 Avena sativa
2.5.1.62 N-Phenylmaleimide since the wild-type enzyme and all other Cys-mutants with the exception of the mutant C304A are inhibited, it is concluded that the inhibitor binds to a non-essential Cys residue to abolish activity Avena sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.62 Mg2+ required, reconstitutes activity after treatment with EDTA Avena sativa
2.5.1.62 Mn2+ partially reconstitutes activity after treatment with EDTA Avena sativa
2.5.1.62 Zn2+ partially reconstitutes activity after treatment with EDTA Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.62 Avena sativa
-
-
-