Literature summary extracted from
Schmid, H.C.; Rassadina, V.; Oster, U.; Schoch, S.; Rüdiger, W.
Pre-loading of chlorophyll synthase with tetraprenyl diphosphate is an obligatory step in chlorophyll biosynthesis (2002), Biol. Chem., 383, 1769-1778.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.5.1.62 |
D147A |
mutant enzyme without activity |
Avena sativa |
2.5.1.62 |
D150A |
mutant enzyme without activity |
Avena sativa |
2.5.1.62 |
D154A |
mutant enzyme without activity |
Avena sativa |
2.5.1.62 |
N146A |
mutant enzyme without activity |
Avena sativa |
2.5.1.62 |
R161A |
mutant enzyme without activity |
Avena sativa |
2.5.1.62 |
R161H |
mutant enzyme with 1% activity compared to wild type enzyme |
Avena sativa |
2.5.1.62 |
R161K |
mutant enzyme with 34% activity compared to wild type enzyme |
Avena sativa |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.62 |
Avena sativa |
- |
recombinant enzyme expressed in Escherichia coli |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.5.1.62 |
chlorophyllide a + phytyl diphosphate = chlorophyll a + diphosphate |
ping-pong mechanism |
Avena sativa |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.62 |
chlorophyllide a + tetraprenyl diphosphate |
ping-pong mechanism. Tetraprenyl diphosphate must bind to the enzyme as the first substrate and esterification occurs when the pre-loaded enzyme meets the second substrate |
Avena sativa |
diphosphate + 3-tetraprenylchlorophyllide a |
- |
? |
|