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Literature summary extracted from

  • Lin, L.L.; Liao, H.F.; Chien, H.R.; Hsu, W.H.
    Identification of essential cysteine residues in 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Corynebacterium glutamicum (2001), Curr. Microbiol., 42, 426-431.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 overexpression of His-tagged wild-type and mutants in Escherichia coli Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.54 C145S site-directed mutagenesis, 16% remaining activity compared to the wild-type, 4.6fold increased Km, 1.6fold decreased kcat, and 13.6fold decreased kcat/Km for phosphoenolpyruvate Corynebacterium glutamicum
2.5.1.54 C334S site-directed mutagenesis, unaltered properties Corynebacterium glutamicum
2.5.1.54 C67L site-directed mutagenesis, highly reduced activity, insensitive to inhibition by divalent metal ions Corynebacterium glutamicum
2.5.1.54 C67S site-directed mutagenesis, inactive Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 Mg2+ no inhibition of mutant C67L; wild-type and mutants Corynebacterium glutamicum
2.5.1.54 Zn2+ no inhibition of mutant C67L; wild-type and mutants Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information mutants Corynebacterium glutamicum
2.5.1.54 0.0043
-
 phosphoenolpyruvate recombinant wild-type, 30°C, pH 6.5 Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ stimulates Corynebacterium glutamicum
2.5.1.54 Co2+ wild-type and mutants, best activator Corynebacterium glutamicum
2.5.1.54 Ni2+ highly activating Corynebacterium glutamicum
2.5.1.54 Ni2+ wild-type and mutants Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinant His-tagged wild-type and mutants from Escherichia coli Corynebacterium glutamicum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate Cys67 and Cys145 are important for the catalytic reaction Corynebacterium glutamicum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 additional information
-
 mutants, influence of aromatic amino acids on activity Corynebacterium glutamicum
2.5.1.54 139.3
-
 recombinant wild-type Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Corynebacterium glutamicum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 30
-
 wild-type and mutants Corynebacterium glutamicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.54 25 50 wild-type and mutants Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information mutants Corynebacterium glutamicum
2.5.1.54 0.0035
-
 phosphoenolpyruvate recombinant wild-type, 30°C, pH 6.5 Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.5
-
 wild-type and mutants Corynebacterium glutamicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 1.5 8.5 wild-type and mutants Corynebacterium glutamicum