Literature summary extracted from
Wagner, T.; Shumilin, I.A.; Bauerle, R.; Kretsinger, R.H.
Structure of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli: Comparison of the Mn2+*2-phosphoglycolate and the Pb2+*2-phosphoenolpyruvate complexes and Implications for catalysis (2000), J. Mol. Biol., 301, 389-399.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.5.1.54 |
Phe-sensitive isozyme, enzyme-Mn2+-2-phosphoglycolate-complexes, hanging drop vapour diffusion method, room temperature, all solutions, except the MnSO4 and the enzyme solution, are treated with Chelex-100 to remove metals, 0.2 mM enzyme subunit solution: 0.37 MnSO4, 4.2 mM 2-phosphoglycolate, 0.1 M Li2SO4, 12% PEG 100 w/v, 20% ethanol v/v, 50 mM 1,3-bis[tris(hydroxy-methyl)methylamino]propane buffer, pH 8.7, reservoir solution: 19% PEG 1000, 0.1 M Li2SO4, 20% ethanol v/v, 50 mM 1,3-bis[tris(hydroxy-methyl)methylamino]propane buffer, X-ray diffraction structure determination and analysis |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.54 |
Escherichia coli |
P0AB91 |
Phe-sensitive isozyme |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.54 |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O |
- |
Escherichia coli |
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.5.1.54 |
More |
active site structure |
Escherichia coli |
2.5.1.54 |
More |
subunit structure |
Escherichia coli |