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Literature summary extracted from

  • Ramilo, C.A.; Evans, J.N.S.
    Overexpression, purification, and characterization of tyrosine-sensitive 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase from Escherichia coli (1997), Protein Expr. Purif., 9, 253-261.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 gene aroF, Tyr-sensitive isozyme, overexpression in strain BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 CN- Tyr-sensitive isozyme, strong inhibition, reactivation by divalent cations only to a small extent Escherichia coli
2.5.1.54 EDTA Mn2+, Cd2+, Co2+, Fe2+, Cu2+, Mg2+ or Zn2+ reactivate, Fe2+ and Cu2+ only partially reactivate Escherichia coli
2.5.1.54 Tyr Tyr-sensitive isozyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information kinetics Escherichia coli
2.5.1.54 additional information
-
 additional information pH-dependence Escherichia coli
2.5.1.54 0.013
-
 phosphoenolpyruvate Tyr-sensitive isozyme, 25°C Escherichia coli
2.5.1.54 0.0814
-
 D-erythrose 4-phosphate Tyr-sensitive isozyme, 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Cd2+ activates, Tyr-sensitive isozyme Escherichia coli
2.5.1.54 Cu2+ activates, Tyr-sensitive isozyme Escherichia coli
2.5.1.54 Fe2+ activates, Tyr-sensitive isozyme Escherichia coli
2.5.1.54 Mg2+ activates, Tyr-sensitive isozyme Escherichia coli
2.5.1.54 Mn2+ Tyr-sensitive isozyme, best activator Escherichia coli
2.5.1.54 Zn2+ activates, Tyr-sensitive isozyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
 tyrosine-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 2fold Escherichia coli
2.5.1.54 recombinant from overexpressing strain Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 additional information
-
-
 Escherichia coli
2.5.1.54 22
-
 purified recombinant Tyr-sensitive isozyme, with Cd2+ Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 29.5
-
 phosphoenolpyruvate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 additional information
-
 Tyr-sensitive isozyme, pIs: 6.1 and 8.9 Escherichia coli
2.5.1.54 7 7.5 recombinant Tyr-sensitive isozyme Escherichia coli