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Literature summary extracted from

  • Sheflyan, G.Y.; Howe, D.L.; Wilson, T.L.; Woodard, R.W.
    Enzymatic synthesis of 3-deoxy-D-manno-octulosonate 8-phosphate 3-deoxy-D-altro-octulosonate 8-phosphate, 3,5-dideoxy-D-gluco(manno)-octulosonate 8-phosphate by 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (1998), J. Am. Chem. Soc., 120, 11027-11032.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 overexpression in BL21(DE3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.005
-
phosphoenolpyruvate recombinant enzyme, with D-arabinose 5-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
2.5.1.54 0.0053
-
phosphoenolpyruvate recombinant enzyme, with erythrose 4-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
2.5.1.54 0.01
-
phosphoenolpyruvate recombinant enzyme, with ribose 5-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
2.5.1.54 0.015
-
phosphoenolpyruvate recombinant enzyme, with 2-deoxyribose 5-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
2.5.1.54 0.03
-
D-arabinose 5-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli
2.5.1.54 0.141
-
D-erythrose 4-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli
2.5.1.54 6
-
ribose 5-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli
2.5.1.54 6.8
-
2-deoxyribose 5-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Escherichia coli first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
phenylalanine-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinant from overexpressing strain Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate
-
Escherichia coli 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-arabinose 5-phosphate
-
Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
ir
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-ribose 5-phosphate
-
Escherichia coli 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 DAH7-P synthase (phe)
-
Escherichia coli