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Literature summary extracted from

  • Paravicini, G.; Schmidheini, T.; Braus, G.
    Purification and properties of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (phenylalanine-inhibitable) of Saccharomyces cerevisiae (1989), Eur. J. Biochem., 186, 361-366.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA Co2+, Mn2+, Zn2+ and Fe2+ restore activity; phosphoenolpyruvate, 2 mM, partially protects from inactivation, does not restore activity Saccharomyces cerevisiae
2.5.1.54 Phe competitive with respect to D-erythrose 4-phosphate and non-competitive to phosphoenolpyruvate; Phe-sensitive isozyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.018
-
 phosphoenolpyruvate Phe-sensitive isozyme, pH 6.8, 30°C Saccharomyces cerevisiae
2.5.1.54 0.13
-
 D-erythrose 4-phosphate Phe-sensitive isozyme, pH 6.8, 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Fe2+ contains 0.6 mol of iron per mol of enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 42130
-
 1 * 42130, calculation from nucleotide sequence Saccharomyces cerevisiae
2.5.1.54 53000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Saccharomyces cerevisiae
-
 Phe-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 Phe-sensitive isozyme, 11fold to homogeneity from overproducing strain Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Saccharomyces cerevisiae 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 monomer 1 * 42130, calculation from nucleotide sequence Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.8
-
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 0.01
-
 Phe Phe-sensitive isozyme, pH 6.8, 30°C Saccharomyces cerevisiae