Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tianhui, X.; Chiao, J.S.
    Purification and properties of DAHP synthase from Nocardia mediterranei (1989), Biochim. Biophys. Acta, 991, 1-5.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 D-erythrose 4-phosphate
-
 Amycolatopsis mediterranei
2.5.1.54 Trp noncompetitive to both substrates; Trp-sensitive isozyme Amycolatopsis mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.25
-
 D-erythrose 4-phosphate Trp-sensitive isozyme, pH 7.2, 37°C Amycolatopsis mediterranei
2.5.1.54 0.4
-
 phosphoenolpyruvate Trp-sensitive isozyme, pH 7.2, 37°C Amycolatopsis mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 35000
-
 4 * 35000, Trp-sensitive isozyme Amycolatopsis mediterranei
2.5.1.54 135000
-
 Trp-sensitive isozyme, gel filtration Amycolatopsis mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Amycolatopsis mediterranei first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Amycolatopsis mediterranei U-32 first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Amycolatopsis mediterranei
-
-
-
2.5.1.54 Amycolatopsis mediterranei
-
 Trp-sensitive isozyme
-
2.5.1.54 Amycolatopsis mediterranei U-32
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 Trp-sensitive isozyme, 130fold Amycolatopsis mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.91
-
 purified enzyme Amycolatopsis mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Amycolatopsis mediterranei 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Amycolatopsis mediterranei 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Amycolatopsis mediterranei U-32 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Amycolatopsis mediterranei U-32 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-fructose 1,6-diphosphate same activity as with D-erythrose 4-phosphate Amycolatopsis mediterranei ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-fructose 6-phosphate same activity as with D-erythrose 4-phosphate Amycolatopsis mediterranei ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-glucose 6-phosphate as effective as D-erythrose 4-phosphate Amycolatopsis mediterranei ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-glucose 6-phosphate as effective as D-erythrose 4-phosphate Amycolatopsis mediterranei U-32 ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-ribose 5-phosphate as effective as D-erythrose 4-phosphate Amycolatopsis mediterranei 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-ribose 5-phosphate as effective as D-erythrose 4-phosphate Amycolatopsis mediterranei U-32 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-xylulose 5-phosphate at 67% of the activity with D-erythrose 4-phosphate Amycolatopsis mediterranei ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-xylulose 5-phosphate at 67% of the activity with D-erythrose 4-phosphate Amycolatopsis mediterranei U-32 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 tetramer 4 * 35000, Trp-sensitive isozyme Amycolatopsis mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.2
-
 Tris-HCl buffer Amycolatopsis mediterranei
2.5.1.54 7.2
-
 phosphate buffer, Trp-sensitive isozyme Amycolatopsis mediterranei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Amycolatopsis mediterranei