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Literature summary extracted from

  • Ip, K.M.; Doy, C.H.
    The kinetic of a purified form of 3-deoxy-D-arabino heptulosonate-7-phosphate synthase (tryptophan) from Neurospora crassa (1979), Eur. J. Biochem., 98, 431-440.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 D-erythrose 4-phosphate substrate-inhibition Neurospora crassa
2.5.1.54 phosphate competitive to D-erythrose 4-phosphate, noncompetitive to phosphoenolpyruvate Neurospora crassa
2.5.1.54 Trp allosteric, best at pH 6.0, no inhibition at pH 7.3; Trp-sensitive isozyme Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Neurospora crassa
-
-
-
2.5.1.54 Neurospora crassa
-
tryptophan-sensitive isozyme
-
2.5.1.54 Neurospora crassa 74A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54
-
Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered ping-pong bi-bi mechanism Neurospora crassa
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate first substrate is phosphoenolpyruvate Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Neurospora crassa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Neurospora crassa 74A 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 additional information
-
phosphoenolpyruvate, D-erythrose 4-phosphate and inorganic phosphate protect the purified enzyme against heat denaturation Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.3
-
tryptophan-sensitive isozyme Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 22
-
phosphate slope, tryptophan-sensitive isozyme, versus phosphoenolpyruvate, pH 7.4, 37°C Neurospora crassa
2.5.1.54 25
-
phosphate slope, tryptophan-sensitive isozyme, versus D-erythrose 4-phosphate, pH 7.4, 37°C Neurospora crassa
2.5.1.54 54
-
phosphate intercept, tryptophan-sensitive isozyme, versus phosphoenolpyruvate, pH 7.4, 37°C Neurospora crassa